ID | 64128 |
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Author |
Isobe, Hiroshi
Research Institute for Interdisciplinary Science, Okayama University
Shoji, Mitsuo
Center for Computational Science, University of Tsukuba,
Suzuki, Takayoshi
Research Institute for Interdisciplinary Science, Okayama University
Shen, Jian-Ren
Research Institute for Interdisciplinary Science, Okayama University
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Yamaguchi, Kizashi
Institute for NanoScience Design, Osaka University
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Abstract | The primary coordination sphere of the multinuclear cofactor (Mn4CaOx) in the oxygen-evolving complex (OEC) of photosystem II is absolutely conserved to maintain its structure and function. Recent time-resolved serial femtosecond crystallography identified large reorganization of the primary coordination sphere in the S-2 to S-3 transition, which elicits a cascade of events involving Mn oxidation and water molecule binding to a putative catalytic Mn site. We examined how the crystallographic fields, created by transient conformational states of the OEC at various time points, affect the thermodynamics of various isomers of the Mn cluster using DFT calculations, with an aim of comprehending the functional roles of the flexible primary coordination sphere in the S-2 to S-3 transition and in the recovery of the S-2 state. The results show that the relative movements of surrounding residues change the size and shape of the cavity of the cluster and thereby affect the thermodynamics of various catalytic intermediates as well as the ability to capture a new water molecule at a coordinatively unsaturated site. The implication of these findings is that the protein dynamics may serve to gate the catalytic reaction efficiently by controlling the sequence of Mn oxidation/reduction and water binding/release. This interpretation is consistent with EPR experiments; g similar to 5 and g similar to 3 signals obtained after near-infrared (NIR) excitation of the S-3 state at 4 K and a g similar to 5 only signal produced after prolonged incubation of the S-3 state at 77 K can be best explained as originating from water-bound S-2 clusters (S-total = 7/2) under a S-3 ligand field, i.e., the immediate one-electron reduction products of the oxyl-oxo (S-total = 6) and hydroxo-oxo (S-total = 3) species in the S-3 state.
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Note | This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpcb.2c02596
This full-text will be available in Sep. 2023.
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Published Date | 2022-09-15
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Publication Title |
The Journal of Physical Chemistry B
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Volume | volume126
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Issue | issue38
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Publisher | American Chemical Society (ACS)
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Start Page | 7212
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End Page | 7228
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ISSN | 1520-6106
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NCID | AA11114073
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022 American Chemical Society
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1021/acs.jpcb.2c02596
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP17H06434
JP18K05146
JP20H05088
JP20H05103
JP22K05317
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