ID | 13423 |
Eprint ID | 13423
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FullText URL | |
Author |
Sakaguchi, Yoshihiko
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Abstract | Clostridium botulinum types C and D toxin production is governed by the specific bacteriophages. A type C phage,c-st,was isolated from a toxigenic strain of C-Stockholm,and its whole genome sequence was determined.The genome was a linear double-stranded 185,681bp DNA with a G+C content of 26.2%, and identical 403-bp sequences were found at each end of the genome. From the DNA research, 198 potential protein-coding regions (ORFs)were identified. The genes for type C progenitor toxin and C3 exoenzyme were identified, but no type D toxin gene was found.A number of genes were homologous to those of a Bacillus subtilis bacteriophage,SP D. On Southern blot analysis between type C and D cell DNAs and the specific probes for H and L chains of C and D
neurotoxins, it became clear that type C and D strains posses only one type of toxin gene.From these findings, it is concluded that cross-neutralization observed between toxins (types C and D)and their antiserums can be caused
by the common-antigens in C and D toxins.
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Keywords | ボツリヌス菌 (Clostridium botulinum)
ファージ (phage)
神経毒素遺伝子 (neurotoxin gene)
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Note | 原著
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Published Date | 2005-05-20
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume117
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Issue | issue1
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 41
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End Page | 46
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/117/1/117_1_41/_article/-char/ja/
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language |
Japanese
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Copyright Holders | Copyright© 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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