ID | 40732 |
FullText URL | |
Title Alternative | Modification by hyperthermia and cepharanthine of the Killing effect of adriamycin on NIH 3T3 cells
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Author |
Mizuta, Akifumi
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Abstract | The cytotoxic effect of anticancer agents depends markedly on the intracellular uptake of the anticancer drug; its maintenance is increased by accelerating the intracellular uptake of anticancer agent and inchbiting. Using with flow-cytometry, we demonstrated that the intracellular uptake of adriamycin (ADM) is increased by hyperthermia, and that cepharanthine (CEP) and Verapamil inhibit its retention. We investigated the cytotoxic effect of the combination of ADR, CEP and hyperthermia, using a NIH3T3 cell line. The cytotoxic effect of ADR is increased by hyperthermia; its effect is acquired at a lower temperature which has no cytotoxic effect alone. With the conbination of CEP, ADR and hyperthermia, the cytotoxic effect is further increased. The synergism is increased by the elevation of temperature and a long of exposure time of CEP. The increase of the intracellular accumulation anticanceragent is important to overcome multidrugresistance, a phenomenon which has that attracted agreat deal of attention.
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Keywords | Adriamycin
cepharantine
加温
殺細胞効果
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Published Date | 1989-04
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume101
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Issue | issue3-4
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 303
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End Page | 314
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/101/3-4/101_3-4_303/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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