ID | 17754 |
Eprint ID | 17754
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FullText URL | |
Title Alternative | CONTRIBUTION OF CELL SURFACE TO SALT TOLERANCE OF STAPHYLOCOCCUS AUREUS -STUDIES BY FREEZE-ETCHING METHOD-
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Author |
Tomochika, Ken-ichi
Sasai, Katsuko
Kanemasa, Yasuhiro
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Abstract | The role of cell surface in a haloresistant mechanism of S. aureus was analyzed by free-ze-etching method. Our comparative studies were carried out on S. aureus cultivated in 0.5% NaCl containing medium (Normal-Staph) and in 10% NaCl containing medium (10% -Staph), as they were or were treated with L-11 enzyme (cell wall lytic enzyme obtained from Flabobacterium sp.). 1. As shown in photographs of non-treated and enzyme-treated cells, both the cell size and cell wall thickness of 10% -Staph increased to about 1.5 times Normal-Staph. 2. When Normal-Staph was exposed to L-11 enzyme, there occurred a gradual decrease in the OD. In the case of 10% -Staph, the OD showed a down-slope with a shoulder which rose in OD once at about 10 minutes after incubation. 3. The surface pattern of plasma membrane of Normal-Staph appeared to be made up of closely arranged spherical subunits. But in the case of 10% -Staph, the spherical subunits were indistinct and appeared to be sparsely located.
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Published Date | 1974-08-30
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume86
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Issue | issue7-8
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 299
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End Page | 307
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/86/7-8/86_7-8_299/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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