FullText URL fulltext.pdf
Author Ogawa, Hirohito| Fujikura, Daisuke| Namba, Hikaru| Yamashita, Nobuko| Honda, Tomoyuki| Yamada, Masao|
Keywords HHV-6B nectin-2 CD112 CD134 virus entry glycoprotein B
Published Date 2022-01-16
Publication Title Viruses-Basel
Volume volume14
Issue issue1
Publisher MDPI
Start Page 160
ISSN 1999-4915
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2022 by the authors.
File Version publisher
PubMed ID 35062364
DOI 10.3390/v14010160
Web of Science KeyUT 000746000400001
Related Url isVersionOf https://doi.org/10.3390/v14010160
FullText URL JVMS81_8_1191.pdf
Author Ogawa, Hirohito| Hirayama, Haruko| Tanaka, Satsuki| Yata, Norio| Namba, Hikaru| Yamashita, Nobuko| Yonemitsu, Kenzo| Maeda, Ken| Mominoki, Katsumi| Yamada, Masao|
Keywords animal experimental facility domestic pig hepatitis E virus zoonosis
Published Date 2019-08-24
Publication Title Journal of Veterinary Medical Science
Volume volume81
Issue issue8
Publisher The Japanese Society of Veterinary Science
Start Page 1191
End Page 1196
ISSN 0916-7250
NCID AA10796138
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders ©2019 The Japanese Society of Veterinary Science
File Version publisher
PubMed ID 31281141
DOI 10.1292/jvms.19-0086
Web of Science KeyUT 000492854900021
Related Url isVersionOf https://doi.org/10.1292/jvms.19-0086
JaLCDOI 10.18926/AMO/53674
FullText URL 69_5_279.pdf
Author Saito, Yukie| Fujii, Yousuke| Yashiro, Masato| Tsuge, Mitsuru| Nosaka, Nobuyuki| Yamashita, Nobuko| Yamada, Mutsuko| Tsukahara, Hirokazu| Morishima, Tsuneo|
Abstract Lung hyperpermeability affects the development of acute respiratory distress syndrome (ARDS), but therapeutic strategies for the control of microvascular permeability have not been established. We examined the effects of edaravone, dexamethasone, and N-monomethyl-L-arginine (L-NMMA) on permeability changes in human pulmonary microvascular endothelial cells (PMVEC) under a hypercytokinemic state. Human PMVEC were seeded in a Boyden chamber. After monolayer confluence was achieved, the culture media were replaced respectively by culture media containing edaravone, dexamethasone, and L-NMMA. After 24-h incubation, the monolayer was stimulated with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Fluorescein-labeled dextran was added. Then the trans-human PMVEC leak was measured. Expressions of vascular endothelial-cadherin (VE-cadherin) and zonula occludens-1 protein (ZO-1) were evaluated using real-time quantitative polymerase chain reaction and immunofluorescence microscopy. The results showed that TNF-α+IL-1β markedly increased pulmonary microvascular permeability. Pretreatment with edaravone, dexamethasone, or L-NMMA attenuated the hyperpermeability and inhibited the cytokine-induced reduction of VE-cadherin expression on immunofluorescence staining. Edaravone and dexamethasone increased the expression of ZO-1 at both the mRNA and protein levels. Edaravone and dexamethasone inhibited the permeability changes of human PMVEC, at least partly through an enhancement of VE-cadherin. Collectively, these results suggest a potential therapeutic approach for intervention in patients with ARDS.
Keywords pulmonary microvascular endothelial cells permeability edaravone vascular endothelial-cadherin zonula occludens-1 protein
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2015-10
Volume volume69
Issue issue5
Publisher Okayama University Medical School
Start Page 279
End Page 290
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 26490025
Web of Science KeyUT 000365519600004
Author Yashiro, Masato| Tsukahara, Hirokazu| Matsukawa, Akihiro| Yamada, Mutsuko| Fujii, Yosuke| Nagaoka, Yoshiharu| Tsuge, Mitsuru| Yamashita, Nobuko| Ito, Toshihiro| Yamada, Masao| Masutani, Hiroshi| Yodoi, Junji| Morishima, Tsuneo|
Published Date 2013-08-01
Publication Title 岡山医学会雑誌
Volume volume125
Issue issue2
Content Type Journal Article
Author Yashiro, Masato| Tsukahara, Hirokazu| Matsukawa, Akihiro| Yamada, Mutsuko| Fujii, Yosuke| Nagaoka, Yoshiharu| Tsuge, Mitsuru| Yamashita, Nobuko| Ito, Toshihiro| Yamada, Masao| Masutani, Hiroshi|
Published Date 2013-01
Publication Title Critical Care Medicine
Volume volume41
Issue issue1
Content Type Journal Article
Author Yamashita, Nobuko|
Published Date 2012-04-01
Publication Title 岡山医学会雑誌
Volume volume124
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/31961
FullText URL fulltext.pdf
Author Yamashita, Nobuko| Kimura, Hiroshi| Morishima, Tsuneo|
Abstract

Epstein-Barr virus (EBV) is usually maintained in an asymptomatic and latent form by the host immune system, and primarily by EBV-specific cytotoxic T cells (CTLs). However, EBV has been linked to several refractory diseases such as EBV-associated hemophagocytic syndrome(EBV-AHS) and chronic active EBV infection (CAEBV). In these ectopic diseases, EBV infects T/NK cells, causing severe immunodeficiency with a very high EBV load. In recent years, the laboratory procedure to assess these types of EBV infections has been improved. In particular, real-time polymerase chain reaction (PCR) has been used to quantify the EBV load, and the MHC: peptide tetramer assay has been used to quantitate EBV-specific CTLs; these tests have been employed for the management of the illnesses associated with EBV infection. Here, we have reviewed the recent progress in the clinical application of these assays. The pathogenesis of EBV-infected T/NK cells, and the host immune response to infection, including the roles carried out by innate immunity and inflammatory cytokines, are likely to be revealed in the future.

Keywords chronic active Epstein-Barr virus infection Epstein-Barr virus-associated hemophagocytic syndrome Real-time PCR tetramer
Amo Type Review
Publication Title Acta Medica Okayama
Published Date 2005-12
Volume volume59
Issue issue6
Publisher Okayama University Medical School
Start Page 239
End Page 246
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 16418766
Web of Science KeyUT 000234176600001
Author Yamashita, Nobuko|
Published Date 2003-06-30
Publication Title
Content Type Thesis or Dissertation