ID | 65038 |
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Author |
Atsumi, Nanako
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yasumatsu, Keiko
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Takashina, Yuriko
School of Pharmaceutical Sciences, Okayama University
Ito, Chiaki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yasui, Norihisa
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
Kaken ID
publons
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Margolskee, Robert F.
Monell Chemical Senses Center
Yamashita, Atsuko
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract | Salt taste sensation is multifaceted: NaCl at low or high concentrations is preferably or aversively perceived through distinct pathways. Cl- is thought to participate in taste sensation through an unknown mechanism. Here, we describe Cl- ion binding and the response of taste receptor type 1 (T1r), a receptor family composing sweet/umami receptors. The T1r2a/T1r3 heterodimer from the medaka fish, currently the sole T1r amenable to structural analyses, exhibited a specific Cl- binding in the vicinity of the amino-acid-binding site in the ligand-binding domain (LBD) of T1r3, which is likely conserved across species, including human T1r3. The Cl- binding induced a conformational change in T1r2a/T1r3LBD at sub- to low-mM concentrations, similar to canonical taste substances. Furthermore, oral Cl- application to mice increased impulse frequencies of taste nerves connected to T1r-expressing taste cells and promoted their behavioral preferences attenuated by a T1r-specific blocker or T1r3 knock-out. These results suggest that the Cl- evokes taste sensations by binding to T1r, thereby serving as another preferred salt taste pathway at a low concentration.
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Keywords | taste receptor
salt taste
chloride
O
latipes
Mouse
Other
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Published Date | 2023-02-28
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Publication Title |
eLife
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Volume | volume12
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Publisher | eLife Sciences Publications Ltd.
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Start Page | e84291
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ISSN | 2050-084X
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © Atsumi, Yasumatsu et al.
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File Version | publisher
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.7554/eLife.84291
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License | https://creativecommons.org/licenses/by/4.0/
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Citation | Nanako Atsumi, Keiko Yasumatsu, Yuriko Takashina, Chiaki Ito, Norihisa Yasui, Robert F Margolskee, Atsuko Yamashita (2023) Chloride ions evoke taste sensations by binding to the extracellular ligand-binding domain of sweet/umami taste receptors eLife 12:e84291 https://doi.org/10.7554/eLife.84291
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP17H03644
JP18H04621
JP20H03195
JP20H04778
JP21H05524
JP20H03855
JP20K02415
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