| FullText URL | fulltext.pdf Fig_1.pptx Fig_2.pptx |
|---|---|
| Author | Maruyama, Yuki| Araki, Motoo| Wada, Koichiro| Yoshinaga, Kasumi| Mitsui, Yosuke| Sadahira, Takuya| Nishimura, Shingo| Edamura, Kohei| Kobayashi, Yasuyuki| Watanabe, Masami| Watanabe, Toyohiko| Monga, Manoj| Nasu, Yasutomo| Kumon, Hiromi| |
| Keywords | urothelial carcinoma urinary tract cancer ureteroscopy long-term survival renal pelvis ureter |
| Note | This is a pre-copyedited, author-produced version of an article accepted for publication in Japanese Journal of Clinical Oncology following peer review. The version of record Long-term ureteroscopic management of upper tract urothelial carcinoma: 28-year single-centre experience, Japanese Journal of Clinical Oncology, Volume 51, Issue 1, January 2021, Pages 130–137 is available online at: https://doi.org/10.1093/jjco/hyaa132.| |
| Published Date | 2020-7-27 |
| Publication Title | Japanese Journal of Clinical Oncology |
| Volume | volume51 |
| Issue | issue1 |
| Publisher | Oxford University Press |
| Start Page | 130 |
| End Page | 137 |
| ISSN | 0368-2811 |
| NCID | AA00690866 |
| Content Type | Journal Article |
| language | 英語 |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 32715306 |
| DOI | 10.1093/jjco/hyaa132 |
| Web of Science KeyUT | 000608420900018 |
| Related Url | isVersionOf https://doi.org/10.1093/jjco/hyaa132 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | La, The Mon| Yamada, Hiroshi| Seiriki, Sayaka| Li, Shun-AI| Fujise, Kenshiro| Katsumi, Natsuho| Abe, Tadashi| Watanabe, Masami| Takei, Kohji| |
| Keywords | endocytosis GluR2 AMPA cortactin MIN6 |
| Published Date | 2020 |
| Publication Title | Cell Structure and Function |
| Volume | volume45 |
| Issue | issue2 |
| Publisher | Japan Society for Cell Biology |
| Start Page | 121 |
| End Page | 130 |
| ISSN | 0386-7196 |
| NCID | AA0060007X |
| Content Type | Journal Article |
| language | 英語 |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2020 The Author(s) |
| File Version | publisher |
| PubMed ID | 32581155 |
| DOI | 10.1247/csf.20020 |
| Web of Science KeyUT | 000587739500003 |
| Related Url | isVersionOf https://doi.org/10.1247/csf.20020 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | La, The Mon| Tachibana, Hiromi| Li, Shun-Ai| Abe, Tadashi| Seiriki, Sayaka| Nagaoka, Hikaru| Takashima, Eizo| Takeda, Tetsuya| Ogawa, Daisuke| Makino, Shin-Ichi| Asanuma, Katsuhiko| Watanabe, Masami| Tian, Xuefei| Ishibe, Shuta| Sakane, Ayuko| Sasaki, Takuya| Wada, Jun| Takei, Kohji| Yamada, Hiroshi| |
| Keywords | dynamin microtubules podocyte primary process |
| Published Date | 2020-10-17 |
| Publication Title | FASEB Journal |
| Volume | volume34 |
| Issue | issue12 |
| Publisher | Wiley |
| Start Page | 16449 |
| End Page | 16463 |
| ISSN | 0892-6638 |
| NCID | AA1066874X |
| Content Type | Journal Article |
| language | 英語 |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2020 The Authors. |
| File Version | publisher |
| PubMed ID | 33070431 |
| NAID | 120006900792 |
| DOI | 10.1096/fj.202001240RR |
| Web of Science KeyUT | 000581167100001 |
| Related Url | isVersionOf https://doi.org/10.1096/fj.202001240RR |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Xu, Naijin| Lin, Wenfeng| Sun, Jingkai| Sadahira, Takuya| Xu, Abai| Watanabe, Masami| Guo, Kai| Araki, Motoo| Li, Gonghui| Liu, Chunxiao| Nasu, Yasutomo| Huang, Peng| |
| Keywords | Nitroxoline Bladder cancer EMT immunotherapy preclinical model |
| Published Date | 2020-09-23 |
| Publication Title | Journal of Cancer |
| Volume | volume11 |
| Issue | issue22 |
| Publisher | Ivyspring International Publisher |
| Start Page | 6633 |
| End Page | 6641 |
| ISSN | 1837-9664 |
| Content Type | Journal Article |
| language | 英語 |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The author(s). |
| File Version | publisher |
| PubMed ID | 33046984 |
| DOI | 10.7150/jca.47025 |
| Web of Science KeyUT | 000575099000017 |
| Related Url | isVersionOf https://doi.org/10.7150/jca.47025 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Noguchi, Hirofumi| Miyagi-Shiohira, Chika| Nakashima, Yoshiki| Kinjo, Takao| Kobayashi, Naoya| Saitoh, Issei| Watanabe, Masami| Shapiro, A. M. James| Kin, Tatsuya| |
| Keywords | induced tissue-specific progenitor cells iTP induced tissue-specific stem cells iTS induced pluripotent stem cells iPSCs reprogramming factors pancreas |
| Published Date | 2019-06-14 |
| Publication Title | Molecular Therapy - Methods and Clinical Development |
| Volume | volume13 |
| Publisher | Cell Press |
| Start Page | 243 |
| End Page | 252 |
| ISSN | 2329-0501 |
| Content Type | Journal Article |
| language | 英語 |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2019 The Author(s). |
| File Version | publisher |
| PubMed ID | 30828587 |
| DOI | 10.1016/j.omtm.2019.01.011 |
| Web of Science KeyUT | 000471284200022 |
| Related Url | isVersionOf https://doi.org/10.1016/j.omtm.2019.01.011 |
| JaLCDOI | 10.18926/AMO/54982 |
|---|---|
| FullText URL | 71_2_135.pdf |
| Author | Mori, Akihiro| Watanabe, Masami| Sadahira, Takuya| Kobayashi, Yasuyuki| Ariyoshi, Yuichi| Ueki, Hideo| Wada, Koichiro| Ochiai, Kazuhiko| Li, Shun-Ai| Nasu, Yasutomo| |
| Abstract | The cluster of differentiation 147 (CD147), also known as EMMPRIN, is a key molecule that promotes cancer progression. We previously developed an adenoviral vector encoding a tumor suppressor REIC/Dkk-3 gene (Ad-REIC) for cancer gene therapy. The therapeutic effects are based on suppressing the growth of cancer cells, but, the underlying molecular mechanism has not been fully clarified. To elucidate this mechanism, we investigated the effects of Ad-REIC on the expression of CD147 in LNCaP prostate cancer cells. Western blotting revealed that the expression of CD147 was significantly suppressed by Ad-REIC. Ad-REIC also suppressed the cell growth of LNCaP cells. Since other researchers have demonstrated that phosphorylated mitogen-activated protein kinases (MAPKs) and c-Myc protein positively regulate the expression of CD147, we investigated the correlation between the CD147 level and the activation of MAPK and c-Myc expression. Unexpectedly, no positive correlation was observed between CD147 and its possible regulators, suggesting that another signaling pathway was involved in the downregulation of CD147. This is the first study to show the downregulation of CD147 by Ad-REIC in prostate cancer cells. At least some of the therapeutic effects of Ad-REIC may be due to the downregulation of the cancer-progression factor, CD147. |
| Keywords | prostate cancer REIC/Dkk-3 CD147 cell growth p38 MAP kinase |
| Amo Type | Original Article |
| Published Date | 2017-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume71 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 135 |
| End Page | 142 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| Copyright Holders | CopyrightⒸ 2017 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 28420895 |
| JaLCDOI | 10.18926/AMO/54508 |
|---|---|
| FullText URL | 70_4_299.pdf |
| Author | Wada, Koichiro| Uehara, Shinya| Ishii, Ayano| Sadahira, Takuya| Yamamoto, Masumi| Mitsuhata, Ritsuko| Takamoto , Atsushi| Araki, Motoo| Kobayashi, Yasuyuki| Watanabe, Masami| Watanabe, Toyohiko| Hotta, Katsuyuki| Nasu, Yasutomo| |
| Abstract | Urinary tract infections (UTIs) are the most common bacterial infections in women, and many patients experience frequent recurrence. The aim of this report is to introduce an on-going prospective phase II clinical trial performed to evaluate the preventive effectiveness of Lactobacillus vaginal suppositories for prevention of recurrent cystitis. Patients enrolled in this study are administered vaginal suppositories containing the GAI 98322 strain of Lactobacillus crispatus every 2 days or 3 times a week for one year. The primary endpoint is recurrence of cystitis and the secondary endpoints are adverse events. Recruitment began in December 2013 and target sample size is 20 participants. |
| Keywords | probiotics lactobacilli Lactobacillus crispatus urinary tract infection vaginal suppository |
| Amo Type | Clinical Study Protocols |
| Published Date | 2016-08 |
| Publication Title | Acta Medica Okayama |
| Volume | volume70 |
| Issue | issue4 |
| Publisher | Okayama University Medical School |
| Start Page | 299 |
| End Page | 302 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| Copyright Holders | CopyrightⒸ 2016 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 27549677 |
| Web of Science KeyUT | 000384748600012 |
| JaLCDOI | 10.18926/AMO/54507 |
|---|---|
| FullText URL | 70_4_295.pdf |
| Author | Araki, Motoo| Wada, Koichiro| Mitsui, Yosuke| Kubota, Risa| Yoshioka, Takashi| Ariyoshi, Yuichi| Kobayashi, Yasuyuki| Kitagawa, Masashi| Tanabe, Katsuyuki| Sugiyama, Hiroshi| Wada, Jun| Watanabe, Masami| Watanabe, Toyohiko| Hotta, Katsuyuki| Nasu, Yasutomo| |
| Abstract | Although graft survival following renal transplantation (RTx) has improved, outcomes following highrisk RTx are variable. Preexisting antibodies, including donor-specific antibodies (DSA), play an important role in graft dysfunction and survival. We have designed a study to investigate the safety and efficacy of anti-CD20 monoclonal antibodies (rituximab) in high-risk RTx recipients. Major eligibility criteria include: 1) major and minor ABO blood group mismatch, 2) positive DSA. Thirty-five patients will receive 200 mg/body of rituximab. The primary endpoint is the incidence of B cell depletion. This study will clarify whether rituximab is efficacious in improving graft survival in high-risk RTx recipients. |
| Keywords | end-stage renal disease immunosuppression kidney transplantation |
| Amo Type | Clinical Study Protocols |
| Published Date | 2016-08 |
| Publication Title | Acta Medica Okayama |
| Volume | volume70 |
| Issue | issue4 |
| Publisher | Okayama University Medical School |
| Start Page | 295 |
| End Page | 297 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| Copyright Holders | CopyrightⒸ 2016 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 27549676 |
| Web of Science KeyUT | 000384748600011 |
| Author | Uchida, Daisuke| Shiraha, Hidenori| Kato, Hironari| Nagahara, Teruya| Iwamuro, Masaya| Kataoka, Junro| Horiguchi, Shigeru| Watanabe, Masami| Takaki, Akinobu| Nouso, Kazuhiro| Nasu, Yasutomo| Yagi, Takahito| Kumon, Hiromi| Yamamoto, Kazuhide| |
|---|---|
| Published Date | 2014-05 |
| Publication Title | Journal of Gastroenterology and Hepatology |
| Volume | volume29 |
| Issue | issue5 |
| Content Type | Journal Article |
| Author | Kurahashi, Hiroaki| Watanabe, Masami| Sugimoto, Morito| Ariyoshi, Yuichi| Mahmood, Sabina| Araki, Motoo| Ishii, Kazushi| Nasu, Yasutomo| Nagai, Atsushi| Kumon, Hiromi| |
|---|---|
| Published Date | 2013-12 |
| Publication Title | Endocrine Journal |
| Volume | volume60 |
| Issue | issue12 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/52144 |
|---|---|
| FullText URL | 68_1_47.pdf |
| Author | Ishikawa, Tsutomu| Araki, Motoo| Hirata, Takeshi| Watanabe, Masami| Ebara, Shin| Watanabe, Toyohiko| Nasu, Yasutomo| Kumon, Hiromi| |
| Abstract | We report 3 patients with the rare complication of an indwelling urethral catheter misdirected into the ureter. This is the largest series to date. Patients were referred to us for a variety of reasons following exchange of their chronic indwelling urinary catheters. CT in all cases demonstrated the urinary catheters residing in the left ureter. The ages of the patients were 37, 67 and 81 years old. All patients suffered from neurogenic bladder. Two patients were female, one was male, and 2 of the 3 had a sensory disorder inhibiting their pain response. The catheters were replaced with open-end Foley catheters. Extensive follow-up CT scans were obtained in one case, demonstrating improvement of hydronephrosis and no evidence of ureteral stenosis. Cystoscopy in this patient demonstrated normally positioned and functioning ureteral orifices. Although the placement of an indwelling urethral catheter is a comparatively safe procedure, one must keep in mind that this complication can occur, particularly in female patients with neurogenic bladder. CT without contrast is a noninvasive, definitive diagnostic tool. |
| Keywords | complication indwelling urethral catheter imaging computed tomography ureter |
| Amo Type | Case Report |
| Published Date | 2014-02 |
| Publication Title | Acta Medica Okayama |
| Volume | volume68 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 47 |
| End Page | 51 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| Copyright Holders | CopyrightⒸ 2014 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 24553489 |
| Web of Science KeyUT | 000331592800008 |
| Author | Huang, P| Kaku, H| Chen, J| Kashiwakura, Y| Saika, T| Nasu, Y| Urata, Y| Fujiwara, T| Watanabe, M| Kumon, H| |
|---|---|
| Published Date | 2010-07 |
| Publication Title | Cancer Gene Therapy |
| Volume | volume17 |
| Issue | issue7 |
| Content Type | Journal Article |
| Author | Sakaguchi, Masakiyo| Kataoka, Ken| Abarzua, Fernando| Tanimoto, Ryuta| Watanabe, Masami| Murata, Hitoshi| Than, Swe Swe| Kurose, Kaoru| Kashiwakura, Yuji| Ochiai, Kazuhiko| Nasu, Yasutomo| Kumon, Hiromi| Huh, Nam-ho| |
|---|---|
| Published Date | 2009-05-22 |
| Publication Title | The Journal of Biological Chemistry |
| Volume | volume284 |
| Issue | issue21 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/48076 |
|---|---|
| FullText URL | 66_1_7.pdf |
| Author | Kawauchi, Keiichiro| Watanabe, Masami| Kaku, Haruki| Huang, Peng| Sasaki, Kasumi| Sakaguchi, Masakiyo| Ochiai, Kazuhiko| Huh, Nam-ho| Nasu, Yasutomo| Kumon, Hiromi| |
| Abstract | The preclinical safety and therapeutic efficacy of adenoviral vectors that express the REIC/Dkk-3 tumor suppressor gene (Ad-REIC) was examined for use in prostate cancer gene therapy. The Ad-human (h) and mouse (m) REIC were previously demonstrated to induce strong anti-cancer effects in vitro and in vivo, and we herein report the results of two in vivo studies. First, intra-tumor Ad-hREIC administration was examined for toxicity and therapeutic effects in a subcutaneous tumor model using the PC3 prostate cancer cell line. Second, intra-prostatic Ad-mREIC administration was tested for toxicity in normal mice. The whole-body and spleen weights, hematological and serum chemistry parameters, and histological evaluation of tissues from throughout the body were analyzed. Both experiments indicated that there was no significant difference in the examined parameters between the Ad-REIC-treated group and the control (PBS- or Ad-LacZ-treated) group. In the in vitro analysis using PC3 cells, a significant apoptotic effect was observed after Ad-hREIC treatment. Confirming this observation, the robust anti-tumor efficacy of Ad-hREIC was demonstrated in the in vivo subcutaneous prostate cancer model. Based on the results of these preclinical experiments, we consider the adenovirus-mediated REIC/Dkk-3 in situ gene therapy to be safe and useful for the clinical treatment of prostate cancer. |
| Keywords | REIC Dickkopf-3 gene therapy prostate cancer preclinical study |
| Amo Type | Original Article |
| Published Date | 2012-02 |
| Publication Title | Acta Medica Okayama |
| Volume | volume66 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 7 |
| End Page | 16 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 22358134 |
| Web of Science KeyUT | 000300800700002 |
| JaLCDOI | 10.18926/AMO/47013 |
|---|---|
| FullText URL | 65_5_315.pdf |
| Author | Wang, Lei| Kaku, Haruki| Huang, Peng| Xu, Kexin| Yang, Kai| Zhang, Jiheng| Li, Ming| Xie, Liping| Wang, Xiaofeng| Sakai, Akiko| Watanabe, Masami| Nasu, Yasutomo| Shimizu, Kenji| Kumon, Hiromi| Na, Yanqun| |
| Abstract | Deficiencies in the human DNA repair gene WRN are the cause of Werner syndrome, a rare autosomal recessive disorder characterized by premature aging and a predisposition to cancer. This study evaluated the association of WRN Leu1074Phe (rs1801195), a common missense single nucleotide polymorphism in WRN, with prostate cancer susceptibility in Chinese subjects. One hundred and forty-seven prostate cancer patients and 111 male cancer-free control subjects from 3 university hospitals in China were included. Blood samples were obtained from each subject, and the single nucleotide polymorphism WRN Leu1074Phe was genotyped by using a Snapshot assay. The results showed that WRN Leu1074Phe was associated with the risk of prostate cancer in Chinese men and that the TG/GG genotype displayed a decreased prevalence of prostate cancer compared with the TT genotype (OR＝0.58, 95%CI:0.35-0.97, p＝0.039). Through stratified analysis, more significant associations were revealed for the TG/GG genotype in the subgroup with diagnosis age <_ 72 yr (OR＝0.27, 95%CI:0.12-0.61, p＝0.002) and in patients with localized diseases (OR＝0.36, 95%CI:0.19-0.70, p＝0.003). However, no statistically significant difference was found in the subgroup with age ＞72 yr or in patients with advanced diseases. We concluded that the genetic variant Leu1074Phe in the DNA repair gene WRN might play a role in the risk of prostate cancer in Chinese subjects. |
| Keywords | polymorphism prostatic neoplasms single nucleotide susceptibility WRN |
| Amo Type | Original Article |
| Published Date | 2011-10 |
| Publication Title | Acta Medica Okayama |
| Volume | volume65 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 315 |
| End Page | 323 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| Copyright Holders | CopyrightⒸ 2011 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 22037267 |
| Web of Science KeyUT | 000296116400005 |
| Author | Ochiai, Kazuhiko| Watanabe, Masami| Ueki, Hideo| Huang, Peng| Fujii, Yasuyuki| Nasu, Yasutomo| Noguchi, Hirofumi| Hirata, Takeshi| Sakaguchi, Masakiyo| Huh, Nam-ho| Kashiwakura, Yuji| Kaku, Haruki| Kumon, Hiromi| |
|---|---|
| Published Date | 2011-08-26 |
| Publication Title | Biochemical and Biophysical Research Communications |
| Volume | volume412 |
| Issue | issue2 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/31964 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nagai, Atsushi| Nasu, Yasutomo| Watanabe, Masami| Kusumi, Norihiro| Tsuboi, Hiromu| Kumon, Hiromi| |
| Abstract | <p>We investigated the usefulness of one-stage urethroplasty by the parameatal foreskin flap method (OUPF procedure), which is useful for repairing all types of hypospadias. Between June 1992 and March 2001, the OUPF procedure was performed on 18 patients with hypospadias: 10 patients with distal and 8 with proximal hypospadias. The follow-up periods ranged from 33-75 months, with an average of 52 months. The duration of surgery, the catheter indwelling period, and the postoperative complications of each patient were analyzed. The median age of the patients at the time of surgery was 3 years and 8 months. The length of surgery for OUPF II ranged from 150-230 min (average 186 min), and from 190-365 min (average 267 min) for OUPF IV. Postoperative complications were confirmed in 3 of the 18 patients (16.6%). Two patients had fistulas, and one had a meatal regression. The fistulas were successfully closed by the simple multilayered closure method. After adopting DuoDerm dressings instead of elastic bandages for protection of the wound, no fistulization occurred. DuoDerm dressings are useful in the healing of wounds without complications. To date, the longest follow-up period has been 75 months, and during that time there have been no late complications such as urethral stenosis or penile curvature. OUPF is a useful method in the treatment of hypospadias with a low incidence of early and late complications.</p> |
| Keywords | hypospadias one-stageure throplasty OUPF DuoDerm dressings |
| Amo Type | Article |
| Published Date | 2005-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume59 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 45 |
| End Page | 48 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 16049554 |
| Web of Science KeyUT | 000228590000002 |
| JaLCDOI | 10.18926/AMO/31681 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Iguchi, Hiroki| Watanabe, Masami| Kamitani, Akihiro| Nagai, Atsushi| Hosoya, Osamu| Tsutsui, Kimiko| Kumon, Hiromi| |
| Abstract | <p>Dynamin is a protein essential to endocytosis. Dynamin 2, a dynamin isoform, is expressed most intensely in testicular tissue; however, precise localization has never been studied. Therefore, we investigated the expression of dynamin 2 in rat testicular tissue using immunohistochemical methods, and discuss here the physiological function of this protein. Testicular tissues were obtained from Wistar rats at 10, 21 and 63 days of age. Immunohistochemistrical examination and Western blot analysis were conducted using dynamin 2 specific antibody. Western blot analysis showed that expression in 21- and 63-day-old rats was more intense than that in 10-day-old rats. Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats. In the 63-day-old rats, the expression was intense, especially in spermatids in the earlier maturation stages and in spermatocytes, and was observed in Sertoli cells. However, in spermatids, the expression gradually declined as spermatids matured to spermatozoa. In the 21-day-old rats, the expression was evident in spermatocytes and Sertoli cells, but that in the 10-day-old rats was weak. Intense expression of dynamin 2 during spermatogenesis suggests that this protein plays an important role in this process.</p> |
| Keywords | dynamin 2 endocytosis spermatogenesis |
| Amo Type | Article |
| Published Date | 2002-08 |
| Publication Title | Acta Medica Okayama |
| Volume | volume56 |
| Issue | issue4 |
| Publisher | Okayama University Medical School |
| Start Page | 205 |
| End Page | 209 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 12199526 |
| Web of Science KeyUT | 000177382600006 |
| JaLCDOI | 10.18926/AMO/30954 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nakanishi, Akira| Abe, Tadashi| Watanabe, Masami| Takei, Kohji| Yamada, Hiroshi| |
| Abstract | <p>Testicular Sertoli cells highly express dynamin 2 and amphiphysin 1. Here we demonstrate that dynamin 2 is implicated in phosphatidylserine (PS)-dependent phagocytosis in Sertoli cells. Immunofluorescence and dual-live imaging revealed that dynamin 2 and amphiphysin 1 accumulate simultaneously at ruffles. These proteins are specifically bound <i>in vitro</i>. Over-expression of dominant negative dynamin 2 (K44A) inhibits liposome-uptake and leads to the mis-localization of amphiphysin 1. Thus, the cooperative function of dynamin 2 and amphiphysin 1 in PS-dependent phagocytosis is strongly suggested.</p> |
| Keywords | dynamin amphiphysin phagocytosis testis |
| Amo Type | Original Article |
| Published Date | 2008-12 |
| Publication Title | Acta Medica Okayama |
| Volume | volume62 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 385 |
| End Page | 391 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| Web of Science KeyUT | 000262025000005 |
| Author | Watanabe, Masami| |
|---|---|
| Published Date | 2007-05-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume119 |
| Issue | issue1 |
| Content Type | Journal Article |
