| ID | 65987 |
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| Author |
Ikawa, Yuka
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Wakai, Takuya
Department of Animal Science, Graduate of Environmental and Life Science, Okayama University
Funahashi, Hiroaki
Department of Animal Science, Graduate of Environmental and Life Science, Okayama University
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Soe, Tet Htut
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Watanabe, Kazunori
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Ohtsuki, Takashi
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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| Abstract | Single-cell-specific delivery of small RNAs, such as short hairpin RNA (shRNA) and small noncoding RNAs, allows us to elucidate the roles of specific upregulation of RNA expression and RNAi-mediated gene suppression in early embryo development. The photoinduced cytosolic dispersion of RNA (PCDR) method that we previously reported can introduce small RNAs into the cytosol of photoirradiated cells and enable RNA delivery into a single-cell in a spatiotemporally specific manner. However, the PCDR method has only been applied to planer cultured cells and not to embryos. This study demonstrated that the PCDR method can be utilized for photo-dependent cytosolic shRNA delivery into a single blastomere and for single blastomere-specific RNA interference in mouse embryos. Our results indicate that PCDR is a promising approach for studying the developmental process of early embryogenesis.
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| Note | The version of record of this article, first published in Scientific Reports, is available online at Publisher’s website: http://dx.doi.org/10.1038/s41598-023-40361-9
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| Published Date | 2023-08-11
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| Publication Title |
Scientific Reports
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| Volume | volume13
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| Issue | issue1
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| Publisher | nature portfolio
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| Start Page | 13050
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| ISSN | 2045-2322
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| Content Type |
Journal Article
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| language |
English
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| OAI-PMH Set |
岡山大学
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| Copyright Holders | © The Author(s) 2023
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| File Version | publisher
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| PubMed ID | |
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| Related Url | isVersionOf https://doi.org/10.1038/s41598-023-40361-9
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| License | http://creativecommons.org/licenses/by/4.0/
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| Citation | Ikawa, Y., Wakai, T., Funahashi, H. et al. Photo-dependent cytosolic delivery of shRNA into a single blastomere in a mouse embryo. Sci Rep 13, 13050 (2023). https://doi.org/10.1038/s41598-023-40361-9
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| Funder Name |
Japan Society for the Promotion of Science
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| 助成番号 | 18H02090
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