このエントリーをはてなブックマークに追加
ID 66565
FullText URL
Author
Chaomulige Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Matsuo, Toshihiko Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ORCID Kaken ID publons researchmap
Sugimoto, Kohei Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Miyaji, Mary Department of Medical Neurobiology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Hosoya, Osamu Department of Medical Neurobiology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Ueda, Masashi Department of Biofunctional Imaging Analysis, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Kobayashi, Ryosuke Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University
Horii, Takuro Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University
Hatada, Izuho Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University
Abstract
Strabismus, a neuro-ophthalmological condition characterized by misalignment of the eyes, is a common ophthalmic disorder affecting both children and adults. In our previous study, we identified the microsomal glutathione S-transferase 2 (MGST2) gene as one of the potential candidates for comitant strabismus susceptibility in a Japanese population. The MGST2 gene belongs to the membrane-associated protein involved in the generation of pro-inflammatory mediators, and it is also found in the protection against oxidative stress by decreasing the reactivity of oxidized lipids. To look for the roles of the MGST2 gene in the development, eye alignment, and overall morphology of the eye as the possible background of strabismus, MGST2 gene knockout (KO) mice were generated by CRISPR/Cas9-mediated gene editing with guide RNAs targeting the MGST2 exon 2. The ocular morphology of the KO mice was analyzed through high-resolution images obtained by a magnetic resonance imaging (MRI) machine for small animals. The morphometric analyses showed that the height, width, and volume of the eyeballs in MGST2 KO homozygous mice were significantly greater than those of wild-type mice, indicating that the eyes of MGST2 KO homozygous mice were significantly enlarged. There were no significant differences in the axis length and axis angle. These morphological changes may potentially contribute to the development of a subgroup of strabismus.
Keywords
comitant strabismus
MGST2 gene
mouse models
genetics
CRISPR/Cas9
PCR
MRI
eye morphology
neuro-ophthalmology
Published Date
2024-02-05
Publication Title
Biomedicines
Volume
volume12
Issue
issue2
Publisher
MDPI
Start Page
370
ISSN
2227-9059
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
Copyright Holders
© 2024 by the authors.
File Version
publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.3390/biomedicines12020370
License
https://creativecommons.org/licenses/by/4.0/
Citation
Chaomulige; Matsuo, T.; Sugimoto, K.; Miyaji, M.; Hosoya, O.; Ueda, M.; Kobayashi, R.; Horii, T.; Hatada, I. Morphometric Analysis of the Eye by Magnetic Resonance Imaging in MGST2-Gene-Deficient Mice. Biomedicines 2024, 12, 370. https://doi.org/10.3390/biomedicines12020370
Funder Name
Japan Agency for Medical Research and Development
助成番号
JP23ama121049