ID | 47701 |
FullText URL | |
Author |
Morooka, Yoshiaki
Oomizu, Souichi
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Abstract | Suckling- and estrogen-induced prolactin release from the anterior pituitary is mediated by alpha-melanocyte stimulating hormone (a-MSH) secreted by the intermediate lobe of the pituitary in the rat. Melanocortin 5-receptors are expressed in the anterior pituitary and probably mediate the alpha-MSH function. In contrast, the mouse anterior pituitary does not express the receptor. To examine whether or not alpha-MSH regulates prolactin release in mice, we performed cell immunoblot assay using anterior pituitary cells from adult female mice. We found that alpha-MSH acted on mammotrophs (prolactin-secreting cells) and stimulated prolactin release in a dose dependent manner. A series of RT-PCR using oligonucleotide primer pairs specific for each subtypes of melanocortin receptors revealed that the melanocortin 3-receptor is the sole receptor expressed in the mouse anterior pituitary. These results suggest that alpha-MSH-induced prolactin release is mediated by melanocortin 3-receptors in female mice.
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Published Date | 1998-08
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Publication Title |
Zoological Science
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Volume | volume15
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Issue | issue4
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Publisher | Zoological Society of Japan
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Publisher Alternative | 日本動物学会
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Start Page | 567
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End Page | 572
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ISSN | 0289-0003
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NCID | AA10545874
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Content Type |
Journal Article
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language |
English
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Copyright Holders | © 1998 Zoological Society of Japan
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File Version | publisher
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Refereed |
True
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DOI | |
PubMed ID | |
Web of Science KeyUT |