ID | 10573 |
Eprint ID | 10573
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FullText URL | |
Title Alternative | 逆転写酵素・DNAポリメラーゼを用いたRT-PCRによる増幅反応で確認されたペッパーマイルドモットルウイルス(PMMoV)感染ピーマンの果実種子におけるウイルスの効果的な不活性化方法
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Author |
Hikichi, Yasufumi
Takeuchi, Shigeharu
Okumura, Ako
Nasu, Yoshiko
Okuno, Tetsuro
Suzuki, Kazumi
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Abstract | A method for efficient inactivation Papper mild mottle virus (PMMoV) in harvested seeds of green pepper was examined based on the infectivity on the leaves of Nicotina glutinosa L.(a local host) and a reverse transcription and polymerase chain reaction (RT-PCR)-based amplification of the viral RNA. The seed homogenates from PMMoV-infected plants produced a large number of necrotic local lesion in N.glutinosa, but soaking the seeds in 10% (w/v) tri-sodium phosphate (Na3PO4) for 20 min or dry sterilization (70C,3h) effectivity eliminated the abundance of PMMoV. However, no necrotic lesion on N.glutinosa or the RT-PCR-basaed amplification was observed with seeds that has been disinfected by Na3PO4 in combination with dry sterilization.
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Keywords | Capsicum annuum L.
pepper mild mottle virus (PMMoV)
RT-PCR
seed disinfection
seed transmission
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Published Date | 2004-02
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Publication Title |
岡山大学農学部学術報告
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Publication Title Alternative | Scientific reports of the Faculty of Agriculture, Okayama University
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Volume | volume93
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Issue | issue1
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Publisher | 岡山大学農学部
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Publisher Alternative | Faculty of Agriculture,Okayama University
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Start Page | 29
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End Page | 32
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ISSN | 0474-0254
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NCID | AN00033029
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Content Type |
Departmental Bulletin Paper
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language |
English
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File Version | publisher
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Refereed |
False
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Eprints Journal Name | srfa
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