ID | 58281 |
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Author |
Hassan, Ghmkin
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
ORCID
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Afify, Said M.
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
ORCID
Nair, Neha
Graduate School of Natural Science and Technology, Okayama University
Kumon, Kazuki
Graduate School of Natural Science and Technology, Okayama University
Osman, Amira
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Du, Juan
Graduate School of Natural Science and Technology, Okayama University
Mansour, Hager
Abu Quora, Hagar A.
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Nawara, Hend M.
Graduate School of Natural Science and Technology, Okayama University
Satoh, Ayano
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Zahra, Maram H.
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Okada, Nobuhiro
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Seno, Akimasa
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Seno, Masaharu
Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Abstract | Cancer stem cells (CSCs) represent the subpopulation of cancer cells with the ability to differentiate into other cell phenotypes and initiated tumorigenesis. Previously, we reported generating CSCs from mouse induced pluripotent stem cells (miPSCs). Here, we investigated the ability of the CSCs to differentiate into hematopoietic cells. First, the primary cells were isolated from malignant tumors that were formed by the CSCs. Non-adherent cells (NACs) that arose from adherent cells were collected and their viability, as well as the morphology and expression of hematopoietic cell markers, were analyzed. Moreover, NACs were injected into the tail vein of busulfan conditioned Balb/c nude mice. Finally, CSCs were induced to differentiate to macrophages while using IL3 and SCF. The round nucleated NACs were found to be viable, positive for hematopoietic lineage markers and CD34, and expressed hematopoietic markers, just like homing to the bone marrow. When NACs were injected into mice, Wright-Giemsa staining showed that the number of white blood cells got higher than those in the control mice after four weeks. CSCs also showed the ability to differentiate toward macrophages. CSCs were demonstrated to have the potential to provide progenies with hematopoietic markers, morphology, and homing ability to the bone marrow, which could give new insight into the tumor microenvironment according to the plasticity of CSCs.
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Keywords | Induced pluripotent stem cells
Cancer stem cells differentiation
tumor microenvironment
hematopoietic cells
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Published Date | 2019-12-29
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Publication Title |
Cancers
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Volume | volume12
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Issue | issue1
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Publisher | MDPI
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Start Page | 82
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ISSN | 2072-6694
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2019 by the authors.
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File Version | publisher
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.3390/cancers12010082
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License | http://creativecommons.org/licenses/by/4.0/
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
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助成番号 | 25242045
18K15243
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