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ID 849
Title Alternative
エンドウプロトプラスト細胞における植物プロモータの発現
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Author
Yamada, Tetsuji
Hashimoto, Tadaaki
Sriprasertsak, Permpong
Kato, Hisaharu
Shiraishi, Tomonori Kakenhi
Oku, Hachiro
Abstract
High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor.
Publication Title
岡山大学農学部学術報告
Published Date
1992
Volume
volume80
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture, Okayama University
Start Page
51
End Page
60
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
OAI-PMH Set
岡山大学
language
英語
File Version
publisher
Eprints Journal Name
srfa