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ID 52140
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Author
Ueno, Tsuyoshi
Toyooka, Shinichi
Fukazawa, Takuya
Kubo, Takafumi
Muraoka, Takayuki
Tanaka, Norimitsu
Maki, Yuho
Furukawa, Masashi
Sakaguchi, Masakiyo Kaken ID researchmap
Yamamoto, Hiromasa ORCID Kaken ID researchmap
Miyoshi, Shinichiro Kaken ID researchmap
Abstract
The microRNA-34s (miR-34s) have p53 response elements in their 5ʼ-flanking regions and demonstrate tumor-suppressive functions. In malignant pleural mesothelioma (MPM), we previously reported that expression of miR-34b and miR-34c (miR-34b/c) was frequently downregulated by methylation in MPM cell lines and primary tumors. The forced overexpression of miR-34b/c showed significant antitumor effects with the induction of apoptosis in MPM cells. In this study, we examined the in vivo antitumor effects of miR-34b/c using adenovirus vector on MPM. We subcutaneously transplanted NCI-H290, a human MPM cell line, into BALB/C mice and injected adenovirus vector expressing miR-34b/c, luciferase driven by the cytomegalovirus promoter (Ad-miR-34b/c or Ad-Luc), or PBS control into tumors over 5mm in diameter. A statistically significant growth inhibition of the tumor volume was observed in the Ad-miR-34b/c group from day 6 onward compared to the Ad-Luc group. The inhibition rate of Ad-miR-34b/c, compared to the tumor volume treated with Ad-Luc, was 58.6% on day 10 and 54.7% on day13. Our results indicate that adenovirus-mediated miR-34b/c gene therapy could be useful for the clinical treatment of MPM.
Keywords
mesothelioma
microRNA
microRNA-34b/c
p53
Amo Type
Original Article
Published Date
2014-02
Publication Title
Acta Medica Okayama
Volume
volume68
Issue
issue1
Publisher
Okayama University Medical School
Start Page
23
End Page
26
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
Copyright Holders
CopyrightⒸ 2014 by Okayama University Medical School
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT