ID | 52140 |
JaLCDOI | |
FullText URL | |
Author |
Ueno, Tsuyoshi
Toyooka, Shinichi
Fukazawa, Takuya
Kubo, Takafumi
Asano, Hiroaki
Kaken ID
Muraoka, Takayuki
Tanaka, Norimitsu
Maki, Yuho
Furukawa, Masashi
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Abstract | The microRNA-34s (miR-34s) have p53 response elements in their 5ʼ-flanking regions and demonstrate tumor-suppressive functions. In malignant pleural mesothelioma (MPM), we previously reported that expression of miR-34b and miR-34c (miR-34b/c) was frequently downregulated by methylation in MPM cell lines and primary tumors. The forced overexpression of miR-34b/c showed significant antitumor effects with the induction of apoptosis in MPM cells. In this study, we examined the in vivo antitumor effects of miR-34b/c using adenovirus vector on MPM. We subcutaneously transplanted NCI-H290, a human MPM cell line, into BALB/C mice and injected adenovirus vector expressing miR-34b/c, luciferase driven by the cytomegalovirus promoter (Ad-miR-34b/c or Ad-Luc), or PBS control into tumors over 5mm in diameter. A statistically significant growth inhibition of the tumor volume was observed in the Ad-miR-34b/c group from day 6 onward compared to the Ad-Luc group. The inhibition
rate of Ad-miR-34b/c, compared to the tumor volume treated with Ad-Luc, was 58.6% on day 10 and 54.7% on day13. Our results indicate that adenovirus-mediated miR-34b/c gene therapy could be useful for the clinical treatment of MPM.
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Keywords | mesothelioma
microRNA
microRNA-34b/c
p53
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Amo Type | Original Article
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Publication Title |
Acta Medica Okayama
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Published Date | 2014-02
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Volume | volume68
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Issue | issue1
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Publisher | Okayama University Medical School
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Start Page | 23
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End Page | 26
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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Copyright Holders | CopyrightⒸ 2014 by Okayama University Medical School
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |