ID | 60114 |
FullText URL | |
Author |
Kataoka, Kensuke
Department of Systems BioMedicine, Tokyo Medical and Dental University
Kurimoto, Ryota
Department of Systems BioMedicine, Tokyo Medical and Dental University
Tsutsumi, Hiroki
Department of Systems BioMedicine, Tokyo Medical and Dental University
Chiba, Tomoki
Department of Systems BioMedicine, Tokyo Medical and Dental University
Kato, Tomomi
Department of Systems BioMedicine, Tokyo Medical and Dental University
Shishido, Kana
Department of Systems BioMedicine, Tokyo Medical and Dental University
Kato, Mariko
Department of Systems BioMedicine, Tokyo Medical and Dental University
Ito, Yoshiaki
Department of Systems BioMedicine, Tokyo Medical and Dental University
Cho, Yuichiro
Anatomy and Physiological Science, Tokyo Medical and Dental University
Hoshi, Osamu
Anatomy and Physiological Science, Tokyo Medical and Dental University
Mimata, Ayako
Research Core, Tokyo Medical and Dental University
Sakamaki, Yuriko
Research Core, Tokyo Medical and Dental University
Nakamichi, Ryo
Department of Systems BioMedicine, Tokyo Medical and Dental University
Lotz, Martin K.
Department of Molecular Medicine, The Scripps Research Institute
Naruse, Keiji
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
Kaken ID
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Asahara, Hiroshi
Department of Systems BioMedicine, Tokyo Medical and Dental University
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Abstract | Tendons and ligaments are pivotal connective tissues that tightly connect muscle and bone. In this study, we developed a novel approach to generate tendon/ligament-like tissues with a hierarchical structure, by introducing the tendon/ligament-specific transcription factor Mohawk (MKX) into the mesenchymal stem cell (MSC) line C3H10T1/2 cells, and by applying an improved three-dimensional (3D) cyclic mechanical stretch culture system. In our developed protocol, a combination of stableMkxexpression and cyclic mechanical stretch synergistically affects the structural tendon/ligament-like tissue generation and tendon related gene expression. In a histological analysis of these tendon/ligament-like tissues, an organized extracellular matrix (ECM), containing collagen type III and elastin, was observed. Moreover, we confirmed thatMkxexpression and cyclic mechanical stretch, induced the alignment of structural collagen fibril bundles that were deposited in a fibripositor-like manner during the generation of our tendon/ligament-like tissues. Our findings provide new insights for the tendon/ligament biomaterial fields.
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Keywords | Mohawk
tendon
ligament
tissue engineering
mechanical-stress
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Published Date | 2020-06-02
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Publication Title |
Frontiers in Cell and Developmental Biology
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Volume | volume8
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Publisher | Frontiers Media
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Start Page | 307
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ISSN | 2296-634X
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2020 Kataoka, Kurimoto, Tsutsumi, Chiba, Kato, Shishido, Kato, Ito, Cho, Hoshi, Mimata, Sakamaki, Nakamichi, Lotz, Naruse and Asahara.
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File Version | publisher
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DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.3389/fcell.2020.00307
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License | https://creativecommons.org/licenses/by/4.0/
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development
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