| ID | 63735 |
| Author |
Sumi, Nanako
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Nagahiro, Shota
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Watanabe, Kazunori
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Ohtsuki, Takashi
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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| Abstract | Tat-U1A-rose bengal conjugate (TatU1A-RB) was prepared as an ultrasound-sensitive RNA carrier molecule. This molecule consists of Tat cell-penetrating peptide, U1A RNA-binding protein, and rose bengal as a sonosensitizer. We demonstrated that TatU1A-RB delivered RNA via the endocytosis pathway, which was followed by ultrasound-dependent endosomal escape and cytosolic dispersion of the RNA. A short hairpin RNA (shRNA) delivered by TatU1A-RB mediated RNA interference (RNAi) ultrasound-dependently. Even by ultrasound irradiation through blood cells, RNAi could be induced with TatU1A-RB and the shRNA. This ultrasound-dependent cytosolic RNA delivery method will serve as the basis for a new approach to nucleic acid therapeutics.
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| Keywords | Ultrasound
Sonosensitizer
Rose Bengal
RNAi
RNA delivery
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| Note | © 2022 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/.
This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.bmcl.2022.128767] .
This fulltext is available in May 2024. |
| Published Date | 2022-7
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| Publication Title |
Bioorganic & Medicinal Chemistry Letters
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| Volume | volume68
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| Publisher | Elsevier BV
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| Start Page | 128767
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| ISSN | 0960-894X
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| NCID | AA1079577X
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| Content Type |
Journal Article
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| language |
English
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| OAI-PMH Set |
岡山大学
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| Copyright Holders | © 2022 Elsevier Ltd.
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| File Version | author
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| PubMed ID | |
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| Related Url | isVersionOf https://doi.org/10.1016/j.bmcl.2022.128767
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| License | http://creativecommons.org/licenses/by-nc-nd/4.0/
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