JaLCDOI | 10.18926/AMO/31855 |
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FullText URL | fulltext.pdf |
Author | Nishibori, Masahiro| Takahashi, Hide K.| Katayama, Hiroshi| Mori, Shuji| Saito, Shinya| Iwagaki, Hiromi| Tanaka, Noriaki| Morita, Kiyoshi| Ohtsuka, Aiji| |
Abstract | Lipopolysaccharide (LPS) is one of the major causes of septic shock. The polymyxin B-immobilized filter column (PMX) was developed for the adsorption of endotoxin by direct hemoperfusion and has been used for the treatment of LPS-induced septic shock. In this study, we demonstrated that PMX also specifically bound monocytes from the peripheral blood leukocytes of septic patients by mean of an analysis of bound cells using immunocytochemical and electron microscopic techniques. The specific removal of monocytes from septic patients may produce beneficial effects by reducing the interaction between monocytes and functionally associated cells including vascular endothelial cells. |
Keywords | septic shock polymixin B-immobilized column monocyte adsorptive removal |
Amo Type | Short Communication |
Publication Title | Acta Medica Okayama |
Published Date | 2009-02 |
Volume | volume63 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 65 |
End Page | 69 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
Web of Science KeyUT | 000263730300009 |
JaLCDOI | 10.18926/AMO/31845 |
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FullText URL | fulltext.pdf |
Author | Wake, Hidenori| Mori, Shuji| Liu, Keyue| Takahashi, Hideo K.| Nishibori, Masahiro| |
Abstract | Angiogenesis involves complex processes mediated by several factors and is associated with inflammation and wound healing. High mobility group box 1 (HMGB1) is released from necrotic cells as well as macrophages and plays proinflammatory roles. In the present study, we examined whether HMGB1 would exhibit angiogenic activity in a matrigel plug assay in mice. HMGB1 in combination with heparin strongly induced angiogenesis, whereas neither HMGB1 nor heparin alone showed such angiogenic activity. The heparin-dependent induction of angiogenesis by HMGB1 was accompanied by increases in the expression of tumor necrosis factor-alpha (TNF-alpha) and vascular endothelial growth factor-A120 (VEGF-A120). It is likely that the dependence of the angiogenic activity of HMGB1 on heparin was due to the efficiency of the diffusion of the HMGB1-heparin complex from matrigel to the surrounding areas. VEGF-A165 possessing a heparin-binding domain showed a pattern of heparin-dependent angiogenic activity similar to that of HMGB1. The presence of heparin also inhibited the degradation of HMGB1 by plasmin in vitro. Taken together, these results suggested that HMGB1 in complex with heparin possesses remarkable angiogenic activity, probably through the induction of TNF-alpha and VEGF-A120. |
Keywords | angiogenesis HMGB1 heparin |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2009-10 |
Volume | volume63 |
Issue | issue5 |
Publisher | Okayama University Medical School |
Start Page | 249 |
End Page | 262 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 19893601 |
Web of Science KeyUT | 000271132000005 |
JaLCDOI | 10.18926/AMO/31812 |
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FullText URL | fulltext.pdf |
Author | Liu, Rui| Mori, Shuji| Wake, Hidenori| Zhang, Jiyong| Liu, Keyue| Izushi, Yasuhisa| Takahashi, Hideo K.| Peng, Bo| Nishibori, Masahiro| |
Abstract | Interaction between the receptor for advanced glycation end products (RAGE) and its ligands has been implicated in the pathogenesis of various inflammatory disorders. In this study, we establish an in vitro binding assay in which recombinant human high-mobility group box 1 (rhHMGB1) or recombinant human S100A12 (rhS100A12) immobilized on the microplate binds to recombinant soluble RAGE (rsRAGE). The rsRAGE binding to both rhHMGB1 and rhS100A12 was saturable and dependent on the immobilized ligands. The binding of rsRAGE to rhS100A12 depended on Ca2 and Zn2, whereas that to rhHMGB1 was not. Scatchard plot analysis showed that rsRAGE had higher affinity for rhHMGB1 than for rhS100A12. rsRAGE was demonstrated to bind to heparin, and rhS100A12, in the presence of Ca2, was also found to bind to heparin. We examined the effects of heparin preparations with different molecular sizesunfractionated native heparin (UFH), low molecular weight heparin (LMWH) 5000Da, and LMWH 3000Da on the binding of rsRAGE to rhHMGB1 and rhS100A12. All 3 preparations concentration-dependently inhibited the binding of rsRAGE to rhHMGB1 to a greater extent than did rhS100A12. These results suggested that heparin's anti-inflammatory effects can be partly explained by its blocking of the interaction between HMGB1 or S100A12 and RAGE. On the other hand, heparin would be a promising effective remedy against RAGE-related inflammatory disorders. |
Keywords | RAGE HMGB1 S100A12 heparin inflammation |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2009-08 |
Volume | volume63 |
Issue | issue4 |
Publisher | Okayama University Medical School |
Start Page | 203 |
End Page | 211 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 19727205 |
Web of Science KeyUT | 000269228400006 |
JaLCDOI | 10.18926/AMO/30306 |
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FullText URL | fulltext.pdf |
Author | Tsutsumi, Koji| Nishibori, Masahiro| Saeki, Kiyomi| |
Abstract | In the presence of extracellular Ca2+, 6,7-dihydro-6,8,8, 10-tetramethyl-8H-pyrano [3, 2-g] chromone-2-carboxylic acid (EAA) had an inhibitory effect on the substance P-induced histamine release from rat peritoneal mast cells. Not only Ca2+ but also Mg2+, Sr2+ and Ba2+ were effective in enhancing the activity of EAA. Marked tachyphylaxis to EAA developed irrespective of the presence or absence of extracellular Ca2+. Cross-tachyphylaxis was observed between EAA and disodium cromoglycate (DSCG). These results indicate that the mode of action of EAA is similar, but not identical, with that of DSCG. |
Keywords | 6 7-dihydro-6 8 8 10-tetramethyl-8H-pyrano(3 2-g) chromone-2-carboxylic acid (EAA) disodium cromoglycate histamine release alkaline-earth metal ions substance P |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 1984-08 |
Volume | volume38 |
Issue | issue4 |
Publisher | Okayama University Medical School |
Start Page | 367 |
End Page | 374 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 6208753 |
Web of Science KeyUT | A1984TG25900006 |
Author | Liu, Keyue| Mori, Shuji| Takahashi, Hideo| Tomono, Yasuko| Wake, Hidenori| Kanke, Toru| Sato, Yasuharu| Hiraga, Norihito| Adachi, Naoto| Yoshino, Tadashi| Nishibori, Masahiro| |
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Published Date | 2008-12-01 |
Publication Title | 岡山医学会雑誌 |
Volume | volume120 |
Issue | issue3 |
Content Type | Journal Article |
Author | 西堀 正洋| |
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Published Date | 2001-08-31 |
Publication Title | 岡山医学会雑誌 |
Volume | volume113 |
Issue | issue2 |
Content Type | Journal Article |
Author | 西堀 正洋| |
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Published Date | 1985-12-31 |
Publication Title | |
Content Type | Thesis or Dissertation |