ID | 63525 |
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Kaneshige, Riku
Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Ohtsuka, Satomi
Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Harada, Yuhei
Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Kawamata, Issei
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Okayama University
Magari, Masaki
Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Kanayama, Naoki
Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Hatano, Naoya
Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Sakagami, Hiroyuki
Department of Anatomy, Kitasato University School of Medicine
Tokumitsu, Hiroshi
Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Abstract | Calcium/calmodulin-dependent protein kinase kinases (CaMKKs) activate CaMKI, CaMKIV, protein kinase B/Akt, and AMP-activated protein kinase (AMPK) by phosphorylating Thr residues in activation loops to mediate various Ca2+-signaling pathways. Mammalian cells expressing CaMKK alpha and CaMKK beta lacking Arg/Pro-rich insert domain (RP-domain) sequences showed impaired phosphorylation of AMPK alpha, CaMKI alpha, and CaMKIV, whereas the autophosphorylation activities of CaMKK mutants remained intact and were similar to those of wild-type CaMKKs. Liver kinase B1 (LKB1, an AMPK kinase) complexed with STRAD and MO25 and was unable to phosphorylate CaMKI alpha and CaMKIV; however, mutant LKB1 with the RP-domain sequences of CaMKK alpha and CaMKK beta inserted between kinase subdomains II and III acquired CaMKI alpha and CaMKIV phosphorylating activity in vitro and in transfected cultured cells. Furthermore, ionomycin-induced phosphorylation of hemagglutinin (HA)-CaMKI alpha at Thr177, HA-CaMKIV at Thr196, and HA-AMPK alpha at Thr172 in transfected cells was significantly suppressed by cotransfection of kinase-dead mutants of CaMKK isoforms, but these dominant-negative effects were abrogated with RP-deletion mutants, suggesting that sequestration of substrate kinases by loss-of-function CaMKK mutants requires the RP-domain. This was confirmed by pulldown experiments that showed that dominant-negative mutants of CaMKK alpha and CaMKK beta interact with target kinases but not RP-deletion mutants. Taken together, these results clearly indicate that both CaMKK isoforms require the RP-domain to recognize downstream kinases to interact with and phosphorylate Thr residues in their activation loops. Thus, the RP-domain may be a promising target for specific CaMKK inhibitors.
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Keywords | AMP-activated protein kinase
Arg/Pro-rich insert domain (RP-domain)
calcium/calmodulin-dependent protein kinase
calcium/calmodulin-dependent protein kinase kinase
substrate recognition
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Note | This is the peer reviewed version of the following article: [Kaneshige, R., Ohtsuka, S., Harada, Y., Kawamata, I., Magari, M., Kanayama, N., Hatano, N., Sakagami, H. and Tokumitsu, H. (2022), Substrate recognition by Arg/Pro-rich insert domain in calcium/calmodulin-dependent protein kinase kinase for target protein kinases. FEBS J, 289: 5971-5984. https://doi.org/10.1111/febs.16467], which has been published in final form at [https://doi.org/10.1111/febs.16467]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages there of by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
This fulltext will be available in May 2023.
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Published Date | 2022-05-17
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Publication Title |
The FEBS Journal
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Volume | volume289
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Issue | issue19
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Publisher | Wiley
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Start Page | 5971
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End Page | 5984
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ISSN | 1742-464X
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NCID | AA11998513
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022 Federation of European Biochemical Societies.
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1111/febs.16467
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP21H02429
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