ID | 65184 |
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Takemoto, Fumiko
Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Uchida-Fukuhara, Yoko
Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kamioka, Hiroshi
Department of Orthodontics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University,
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Okamura, Hirohiko
Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Ikegame, Mika
Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract | Osteoblasts alignment and migration are involved in the directional formation of bone matrix and bone remodeling. Many studies have demonstrated that mechanical stretching controls osteoblast morphology and alignment. However, little is known about its effects on osteoblast migration. Here, we investigated changes in the morphology and migration of preosteoblastic MC3T3-E1 cells after the removal of continuous or cyclic stretching. Actin staining and time-lapse recording were performed after stretching removal. The continuous and cyclic groups showed parallel and perpendicular alignment to the stretch direction, respectively. A more elongated cell morphology was observed in the cyclic group than in the continuous group. In both stretch groups, the cells migrated in a direction roughly consistent with the cell alignment. Compared to the other groups, the cells in the cyclic group showed an increased migration velocity and were almost divided in the same direction as the alignment. To summarize, our study showed that mechanical stretching changed cell alignment and morphology in osteoblasts, which affected the direction of migration and cell division, and velocity of migration. These results suggest that mechanical stimulation may modulate the direction of bone tissue formation by inducing the directional migration and cell division of osteoblasts.
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Keywords | Cell alignment
Cell division
Mechanical stress
Migration
Osteoblasts
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Note | The version of record of this article, first published in Anatomical Science International, is available online at Publisher’s website: http://dx.doi.org/10.1007/s12565-023-00716-8
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Published Date | 2023-04-06
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Publication Title |
Anatomical Science International
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Publisher | Springer Science and Business Media LLC
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ISSN | 1447-6959
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © The Author(s) 2023
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1007/s12565-023-00716-8
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License | http://creativecommons.org/licenses/by/4.0/
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Citation | Takemoto, F., Uchida-Fukuhara, Y., Kamioka, H. et al. Mechanical stretching determines the orientation of osteoblast migration and cell division. Anat Sci Int (2023). https://doi.org/10.1007/s12565-023-00716-8
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | 19K07269
22K06790
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