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ID 32987
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Author
Muraguchi, Hajime
Kamada, Takashi
Abstract

The formation of the pileus in homobasidiomycete fungi is essential for sexual reproduction, because the pileus bears the hymenium, a layer of cells that includes the specialised basidia in which nuclear fusion, meiosis and sporulation occur. The developmental mutant ichijiku of Coprinus cinereus fails to develop a differentiated pileus at the apex of the primordial shaft, which is the basal part of the fruit-body primordia and formed in an early stage of fruit-body differentiation. Genetic analysis indicates that this phenotype is caused by a recessive mutation in a single gene (ich1). The ich1 gene was mapped to chromosome XII using restriction fragment length polymorphism markers and the marker chromosome method, and cloned by complementation using a chromosome-XII-specific cosmid library. The ich1 gene encodes a novel protein of 1,353 amino acids. The Ich1 amino-acid sequence contains nuclear targeting signals, suggesting that the Ich1 protein would function in the nucleus. Northern blot analysis indicates that the ich1 gene is specifically expressed in the pileus of the wild-type fruit-body. No ich1 mRNA was detected in the ichijiku mutant, consistent with loss of the promoter region of ich1 in the mutant genome. These data demonstrate that the ick1 gene product is essential for pileus formation.

Keywords
Developmental mutant
Ich1
Pileus
Fruit-body
morphogenesis
Basidiomycete
Coprinus
Note
Published with permission from the copyright holder. This is the institute's copy, as published in Development, 15 August 1998, Volume 125, Issue 16, Pages 3133-3141.
Publisher URL:http://dev.biologists.org/cgi/reprint/125/16/3133
Direct access to Thomson Web of Science record
Copyright 1998 © The Company of Biologists Ltd. All rights reserved.
Published Date
1998-8
Publication Title
Development
Volume
volume125
Issue
issue16
Start Page
3133
End Page
3141
Content Type
Journal Article
language
英語
Refereed
True
DOI
Web of Science KeyUT
Submission Path
biology_general/4