ID | 65507 |
FullText URL | |
Author |
Akiyama, Mari
Department of Child Neurology, Okayama University Hospital
Akiyama, Tomoyuki
Department of Paediatrics (Child Neurology), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Saigusa, Daisuke
Tohoku Medical Megabank Organization, Tohoku University
Hishinuma, Eiji
Tohoku Medical Megabank Organization, Tohoku University
Matsukawa, Naomi
Tohoku Medical Megabank Organization, Tohoku University
Shibata, Takashi
Department of Child Neurology, Okayama University Hospital
Tsuchiya, Hiroki
Department of Child Neurology, Okayama University Hospital
Mori, Atsushi
Department of Neurology, Shiga Medical Centre for Children
Fujii, Yuji
Department of Paediatrics, Hiroshima City Funairi Citizens Hospital
Mogami, Yukiko
Department of Paediatric Neurology, Osaka Women's and Children's Hospital
Tokorodani, Chiho
Department of Paediatrics, Kochi Health Sciences Centre
Kuwahara, Kozue
Department of Paediatrics, Ehime Prefectural Central Hospital,
Numata-Uematsu, Yurika
Department of Paediatrics, Tohoku University School of Medicine
Inoue, Kenji
Department of Neurology, Shiga Medical Centre for Children
Kobayashi, Katsuhiro
Department of Paediatrics (Child Neurology), Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Abstract | Objective: The ketogenic diet (KD), a high-fat and low-carbohydrate diet, is effective for a subset of patients with drug-resistant epilepsy, although the mechanisms of the KD have not been fully elucidated. The aims of this observational study were to investigate comprehensive short-term metabolic changes induced by the KD and to explore candidate metabolites or pathways for potential new therapeutic targets.
Methods: Subjects included patients with intractable epilepsy who had undergone the KD therapy (the medium-chain triglyceride [MCT] KD or the modified Atkins diet using MCT oil). Plasma and urine samples were obtained before and at 2–4 weeks after initiation of the KD. Targeted metabolome analyses of these samples were performed using gas chromatography-tandem mass spectrometry (GC/MS/MS) and liquid chromatography-tandem mass spectrometry (LC/MS/MS). Results: Samples from 10 and 11 patients were analysed using GC/MS/MS and LC/MS/MS, respectively. The KD increased ketone bodies, various fatty acids, lipids, and their conjugates. In addition, levels of metabolites located upstream of acetyl-CoA and propionyl-CoA, including catabolites of branched-chain amino acids and structural analogues of γ-aminobutyric acid and lactic acid, were elevated. Conclusions: The metabolites that were significantly changed after the initiation of the KD and related metabolites may be candidates for further studies for neuronal actions to develop new anti-seizure medications. |
Keywords | Amino acids
Biomarkers
Intractable epilepsy
Ketone bodies
Organic acids
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Note | © 2023 British Epilepsy Association. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/.
This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.seizure.2023.03.014.
This fulltext file will be available in Apr. 2024.
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Published Date | 2023-04
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Publication Title |
Seizure
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Volume | volume107
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Publisher | Elsevier BV
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Start Page | 52
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End Page | 59
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ISSN | 1059-1311
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NCID | AA1091615X
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2023 British Epilepsy Association.
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File Version | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1016/j.seizure.2023.03.014
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License | http://creativecommons.org/licenses/by-nc-nd/4.0/
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Funder Name |
Japan Epilepsy Research Foundation
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development
Japan Society for the Promotion of Science
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助成番号 | JERF TENKAN 19001
JP20km0105001
JP21tm0124005
JP20H03374
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