ID | 64142 |
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Author |
Tani, Yumeki
Department of Chemistry, Okayama University
Ochiai, Kenta
Department of Chemistry, Okayama University
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Abstract | Extracellular vesicles (EVs) exist in biological fluids such as blood, urine, and cerebrospinal fluid and are promising cancer biomarkers. Attempts to isolate and analyze trace EVs, however, have been a challenge for researchers studying their functions and secretion mechanisms, which has stymied the options for diagnostic application. This study demonstrated a collection of EVs that was enhanced by gold nanoparticles (AuNPs) via the use of optical force. The collection system consists of an inverted microscope equipped with a CCD camera, a square capillary connected with a PTFE tube, and an Nd:YAG laser that generates optical force. The laser beam was focused on a capillary wall in which a cell culture medium containing EVs flowed continuously. Control of the surface charges on both the capillary wall and the AuNPs achieved the collection and retention of EVs on the capillary wall. The positively charged capillary wall retained EVs even after the laser irradiation was halted due to the negative charges inherent on the surface of EVs. Conversely, positively charged AuNPs had a strong electrostatic interaction with EVs and enhanced the optical force acting on them, which made collecting them a much more efficient process.
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Keywords | Optical force
Extracellular vesicle
exosome
Gold nanoparticle
Optical trapping
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Note | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s44211-022-00207-2
This full-text will be available in November 2023.
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Published Date | 2022-11-05
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Publication Title |
Analytical Sciences
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Volume | volume39
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Issue | issue5
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Publisher | Springer Science and Business Media LLC
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Start Page | 643
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End Page | 651
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ISSN | 0910-6340
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022, The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry
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File Version | author
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DOI | |
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Related Url | isVersionOf https://doi.org/10.1007/s44211-022-00207-2
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Citation | Tani, Y., Ochiai, K. & Kaneta, T. Optical collection of extracellular vesicles in a culture medium enhanced by interactions with gold nanoparticles. ANAL. SCI. 39, 643–651 (2023). https://doi.org/10.1007/s44211-022-00207-2
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP19H04675
JP20H02766
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