ID | 57784 |
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Boonpoempoon, Tuchpongpuch
Department of Chemistry, Faculty of Science and Technology, Suan Sunandha Rajabhat University
Wonsawat, Wanida
Department of Chemistry, Faculty of Science and Technology, Suan Sunandha Rajabhat University
Kaneta, Takashi
Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
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Abstract | Stabilizing reagents that can be deposited onto paper is an important issue for researchers who depend on paper-based analytical devices (PADs), because long-term stability of the devices is essential in pointof-care testing. Here, we found that poly(vinyl alcohol) (PVA) would stabilize hydrogen peroxide placed on a paper substrate following exposure to air. Horseradish peroxidase was employed as a sample in colorimetric measurements of PADs after hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine were deposited as substrates in an enzymatic reaction. The addition of PVA to hydrogen peroxide significantly suppressed its degradation. Concentrations of PVA that ranged from 0.5 to 2%, increased the duration of the stability of hydrogen peroxide, and the results for a PVA concentration of 1% approximated those of 2% PVA. Storage of the PADs at 4 degrees C in a refrigerator extended the stability of the hydrogen peroxide containing 2% PVA by as much as 30 days. The stability of hydrogen peroxide without PVA was degraded after one day under room temperature.
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Published Date | 2019-9-10
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Publication Title |
Scientific Reports
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Volume | volume9
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Publisher | Nature Publishing Group
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Start Page | 12951
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ISSN | 2045-2322
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © Te Author(s) 2019
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1038/s41598-019-49393-6
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License | https://creativecommons.org/licenses/by/4.0/
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Citation | Boonpoempoon, T., Wonsawat, W. & Kaneta, T. Long-term stabilization of hydrogen peroxide by poly(vinyl alcohol) on paper-based analytical devices. Sci Rep 9, 12951 (2019) doi:10.1038/s41598-019-49393-6
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
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助成番号 | JP17H05465
JP19H04675
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Open Access (Publisher) |
OA
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