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ID 57498
Author
Ichinose, Yuki Graduate School of Environmental and Life ScienceOkayama University Kakenhi
Tasaka, Yousuke Graduate School of Environmental and Life ScienceOkayama University
Yamamoto, Satoru Graduate School of Environmental and Life ScienceOkayama University
Inoue, Yuko Faculty of AgricultureOkayama University
Takata, Motohiro Graduate School of Environmental and Life ScienceOkayama University
Nakatsu, Yukiko Graduate School of Environmental and Life ScienceOkayama University
Taguchi, Fumiko Graduate School of Environmental and Life ScienceOkayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life ScienceOkayama University
Toyoda, Kazuhiro Graduate School of Environmental and Life ScienceOkayama University
Noutoshi, Yoshiteru Graduate School of Environmental and Life ScienceOkayama University
Matsui, Hidenori Graduate School of Environmental and Life ScienceOkayama University
Abstract
Quorum sensing (QS) is a mechanism for bacterial cell-cell communication using QS signals. N-acyl-homoserine lactones (AHLs), QS signals in Pseudomonas syringae pv. tabaci (Pta) 6605, are synthesized by an AHL synthase (PsyI) and recognized by the cognate transcription factor PsyR. To reveal the role of PsyR in virulence, we generated a psyR mutant and complemented strains of Pta 6605 and found that the psyR mutant is remarkably reduced in AHL production and ability to cause disease and propagate in host tobacco leaves. The phenotypes of complemented strains were restored to that of the wild type (WT). Because the psyR mutant lost nearly all AHL production, we investigated the function of PsyR in the transcription of psyI and production of AHL. Electrophoretic mobility shift assays suggested that the recombinant PsyR protein binds the promoter region of psyI but not psyR without AHL. The addition of AHL did not significantly affect this binding. The binding core sequence of this region was identified as a 20-bp lux box-like sequence. To reveal the function of PsyR and AHL on psyI transcription, we constructed a psyI promoter::lacZYA chimeric reporter gene, and inserted it into the WT and psyI mutant of Pta 6605. beta-galactosidase activity increased in a bacterial density-dependent manner in the WT and also in a psyI mutant after the addition of exogenous AHL. These results indicate that the solo PsyR binds the lux box in the psyI promoter and activates transcription in the concomitant presence of AHL.
Keywords
AHL
PsyI
PsyR
Quorum sensing
Note
This fulltext will be available in Nov 2020
Published Date
2019-11-11
Publication Title
Journal of General Plant Pathology
Publisher
Springer
Start Page
1
End Page
10
ISSN
13452630
NCID
AA11449093
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
File Version
author
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1007/s10327-019-00893-3
Citation
Ichinose, Y., Tasaka, Y., Yamamoto, S. et al. J Gen Plant Pathol (2019). https://doi.org/10.1007/s10327-019-00893-3
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
15H04458
19H02956