ID | 62444 |
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Author |
Ho, Dung Thi
Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University
Pham, Hung Hoang Son
Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University
Aota, Wataru
Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University
Matsubayashi, Makoto
Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
Tsuji, Naotoshi
Department of Parasitology and Tropical Medicine, Kitasato University School of Medicine
Hatabu, Toshimitsu
Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University
Kaken ID
researchmap
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Abstract | This study aimed to evaluate the disease severity and local immune responses in macrophage-depleted chicks with Eimeria tenella. Macrophages were reduced by intraperitoneal injection of a carrageenan solution at 12, 13, and 16 days old, whereas the control group received intraperitoneal phosphate-buffered saline. Both chick groups were orally inoculated with E. tenella sporulated oocysts at 14 days old. Feces were collected daily, which were then quantified for oocysts. The chicks were sacrificed on day 5, and the ceca were collected for histopathological observation. The gene expression levels were measured using real-time quantitative reverse transcription-polymerase chain reaction. Macrophage-depleted chicks have been observed to shed a significantly reduced number of fecal oocysts compared to the infected control group. The parasite burden score in cecum specimens of macrophage-depleted chicks was significantly lower than those of infected control on day 5 after infection. Furthermore, macrophage reduction yielded significantly lower cecum histopathological scores and CD4 expression than those of the infected control group. The expression of interleukin (IL)-18, IL-22, interferon-γ, and inducible nitric oxide synthase was also noted to be significantly upregulated in both infected control and macrophage-depleted chicks compared to uninfected chicks. IL-4, IL-13, IL-17, and perforin expressions were also higher with macrophage depletion than in both control groups. These results suggest that macrophages serve as an invasive gate or a transporting vehicle to the site of first merogony. Furthermore, mononuclear phagocytes may play an important role in local immune responses, thus contributing to parasite development during early E. tenella infection.
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Keywords | Eimeria tenella
Local immune response
Macrophage
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Note | © 2021 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 License.http://creativecommons.org/licenses/by-nc-nd/4.0/.This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.rvsc.2021.07.003.
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Published Date | 2021-10
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Publication Title |
Research in Veterinary Science
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Volume | volume139
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Publisher | Elsevier BV
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ISSN | 0034-5288
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NCID | AA00816614
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2021 Elsevier Ltd.
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1016/j.rvsc.2021.07.003
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License | http://creativecommons.org/licenses/by-nc-nd/4.0/
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Citation | Ho DT, Pham HHS, Aota W, Matsubayashi M, Tsuji N, Hatabu T. Reduction of macrophages by carrageenan decreases oocyst output and modifies local immune reaction in chick cecum with Eimeria tenella. Res Vet Sci. 2021 Oct;139:59-66.
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Funder Name |
Research Grant for Encouragement of Students, Graduate School of Environmental and Life Science, Okayama University
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Open Access (Publisher) |
non-OA
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