ID | 910 |
Eprint ID | 910
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FullText URL | |
Title Alternative | 胚珠培養、胚培養による3倍体マスカット個体の育成
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Author |
Okamoto, Goro
Tanakamaru, Naomi
Omori, Naoki
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Abstract | To breed new seedless grape cultivars we crossed 'Muscat of Alexandria' (2x) and its 4x mutant reciprocally and cultured the immature ovules and embryos in vitro. Ovules sampled 50 days after crossing could develop embryos on a liquid medium of half strength of MS supplemented with 2 ppm of IAA, 0.4 ppm of GA3 and 0.1 % of activated charcoal. After 55 days culture, normally developed embryos were obtained from 20 to 30 % of the ovules from 4x×2x and 50 to 70 % of those from 2x×4x. Embryos taken out from the cultured ovules were planted on an agar medium of MS or N&N for 55 to 65 days. Ten to twenty % of embryos from 2x×4x crossing grew to rooted plantlets successfully. They were proved to be triploid after the measurement of chromosome number. Embryos from 4x×x2x, however, could not grow to plantlets because of the failure of rooting.
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Keywords | 胚珠培養
胚培養
マスカット個体
育成
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Published Date | 1993
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Publication Title |
岡山大学農学部学術報告
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Publication Title Alternative | Scientific Reports of the Faculty of Agriculture Okayama University
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Volume | volume82
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Issue | issue1
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Publisher | 岡山大学農学部
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Publisher Alternative | Faculty of Agriculture, Okayama University
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Start Page | 25
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End Page | 29
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ISSN | 0474-0254
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NCID | AN00033029
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Content Type |
Departmental Bulletin Paper
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language |
English
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File Version | publisher
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Refereed |
False
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Eprints Journal Name | srfa
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