ID | 48823 |
FullText URL | |
Author |
Koreishi, Mayuko
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Abstract | Gene perturbation methods are commonly used in the study of gene and protein function. The authors of this paper recently developed a rapid protein inactivation technique utilizing tobacco etch virus (TEV)-derived protease. TEV protease recognizes the ENLYFQG (Glu-Asn-Leu-Tyr-Phe-Gln-Gly) amino acid sequence and specifically cleaves between Q and G. The authors developed antibodies that recognize the cleaved TEV (ENLYFQ) sequence, both in vitro and in vivo, but do not bind to uncleaved TEV (ENLYFQG). Using these antibodies, in situ protein cleavage was successfully detected. These antibodies used in combination with the TEV protease may be a useful complement to other perturbation methods.
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Keywords | TEV protease
Golgi
golgins
microinjection
recombinant proteins
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Published Date | 2011-08-09
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Publication Title |
Antibody Technology Journal
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Volume | volume2011
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Issue | issue1
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Publisher | Dove Medical Press
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Start Page | 5
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End Page | 11
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ISSN | 2230-3170
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Content Type |
Journal Article
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Official Url | http://www.dovepress.com/detection-of-in-situ-cleaved-p115-with-the-cut-specific-antibodies-in--peer-reviewed-article-ANTI
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language |
English
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Copyright Holders | © 2011 Koreishi et al
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File Version | publisher
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Refereed |
True
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DOI | |
NAID |