| ID | 60542 |
| FullText URL | |
| Author |
Abe, Fumitaka
Division of Basic Research, Institute of Crop Science, NARO
Ishida, Yuji
Plant Innovation Center, Japan Tobacco Inc.
Hisano, Hiroshi
Institute of Plant Science and Resources, Okayama University
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Endo, Masaki
Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
Komari, Toshihiko
Plant Innovation Center, Japan Tobacco Inc.
Toki, Seiichi
Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
Sato, Kazuhiro
Institute of Plant Science and Resources, Okayama University
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| Abstract | Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for mutant production. The mutants produced by this protocol can be used for the analysis of gene function and crop improvement. For complete details on the use and execution of this protocol, please refer to Abe et al. (2019).
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| Published Date | 2020-06-13
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| Publication Title |
STAR Protocols
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| Volume | volume1
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| Issue | issue2
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| Publisher | Cell Press
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| Start Page | 100053
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| ISSN | 2666-1667
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| Content Type |
Journal Article
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| language |
English
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| OAI-PMH Set |
岡山大学
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| Copyright Holders | © 2020 The Author(s).
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| File Version | publisher
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| DOI | |
| Related Url | isVersionOf https://doi.org/10.1016/j.xpro.2020.100053
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| License | https://creativecommons.org/licenses/by/4.0/
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| Open Access (Publisher) |
OA
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| Open Archive (publisher) |
Non-OpenArchive
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