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ID 32275
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Author
Harada, Masakazu
Matsuoka, Hiroyuki
Ishii, Akira
Suguri, Setsuo
Abstract

We developed a sensitive polymerase chain reaction (PCR) method for the detection of Plasmodium falciparum DNA from mosquitoes collected in the field. Plasmodium falciparum was detected from 15.2% of 1-parous mosquitoes, Anopheles farauti, in the Solomon Islands through use of the PCR method. A novel mathematical model was developed to estimate the sporozoite rate based on the malaria-positive rate of 1-parous mosquitoes. Using this model, the sporozoite rate of Anopheles farauti in the Solomon Islands was calculated to be 0.09%. This method enables estimation of the sporozoite rate based on a relatively small number (100-200) of mosquitoes compared with the number needed for the ELISA method.

Keywords
sporozoite rate
polymerase chain reaction (PCR)
mathematical model
Anopheles
Plasmodium
Amo Type
Article
Published Date
2000-08
Publication Title
Acta Medica Okayama
Volume
volume54
Issue
issue4
Publisher
Okayama University Medical School
Start Page
165
End Page
171
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT