JaLCDOI 10.18926/AMO/48687
FullText URL 66_4_329.pdf
Author Matsushita, Koki| Mizushima, Takaaki| Shirahige, Akinori| Tanioka, Hiroaki| Sawa, Kiminari| Ochi, Koji| Tanimoto, Mitsune| Koide, Norio|
Abstract The relationship between pancreatic fibrosis and apoptosis of pancreatic acinar cells has not been fully elucidated. We reported that taurine had an anti-fibrotic effect in a dibutyltin dichloride (DBTC)-chronic pancreatitis model. However, the effect of taurine on apoptosis of pancreatic acinar cells is still unclear. Therefore, we examined apoptosis in DBTC-chronic pancreatitis and in the AR42J pancreatic acinar cell line with/without taurine. Pancreatic fibrosis was induced by a single administration of DBTC. Rats were fed a taurine-containing diet or a normal diet and were sacrificed at day 5. The AR42J pancreatic acinar cell line was incubated with/without DBTC with taurine chloramines. Apoptosis was determined by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay. The expression of Bad and Bcl-2 proteins in the AR42J cells lysates was detected by Western blot analysis. The apoptotic index of pancreatic acinar cells in DBTC-administered rats was significantly increased. Taurine treatment inhibited pancreatic fibrosis and apoptosis of acinar cells induced by DBTC. The number of TUNEL-positive cells in the AR42J pancreatic acinar cell lines was significantly increased by the addition of DBTC. Incubation with taurine chloramines ameliorated these changes. In conclusion, taurine inhibits apoptosis of pancreatic acinar cells and pancreatitis in experimental chronic pancreatitis.
Keywords apoptosis chronic pancreatitis pancreatic acinar cells taurine
Amo Type Original Article
Published Date 2012-08
Publication Title Acta Medica Okayama
Volume volume66
Issue issue4
Publisher Okayama University Medical School
Start Page 329
End Page 334
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22918205
Web of Sience KeyUT 000307918900005
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/49739
JaLCDOI 10.18926/AMO/48563
FullText URL 66_3_239.pdf
Author Sawa, Kiminari| Mizushima, Takaaki| Matsushita, Koki| Shirahige, Akinori| Ochi, Koji| Koide, Norio|
Abstract Plain abdominal radiography is a very basic examination and plays an important role in primary care. The objectives of this study were to clarify colon distributions on plain abdominal radiographs. Forty-three healthy volunteers underwent gastric fluoroscopy, and 2 hours later, plain abdominal radiography in the supine position. A region of interest (ROI) was defined uniformly on each X-ray image to divide the image into 600 zones. The area corresponding to the large bowel within the ROI was divided into 4 segments (ascending colon, transverse colon, descending colon, and sigmoid colon+rectum). The percentage of barium in each segment relative to the total volume of barium used was calculated to evaluate the percent ROI occupancy. The large bowel covered 76.7% of the entire ROI, with the percent duplication being 55%. The duplicated area corresponded to the transverse colon region. When the method proposed by Arhan et al. was used, the percentage of the colon actually present in each segment relative to that determined theoretically was 99.6% for the right colon segment, 92.2% for the left colon segment, and 92.2% for the sigmoid/rectal segment. However, in cases in which the transverse colon descended partially from the fifth lumbar vertebra, the percentage occupied by the sigmoid colon+rectum decreased to 57.2%. We applied a new large bowel segmentation method especially for patients with ptosis, by devising a line joining the lateral side of the right lesser pelvis and the lower ends of both sacroiliac joints.
Keywords large bowel segmentation plain abdominal radiograph classification method primary care
Amo Type Original Article
Published Date 2012-06
Publication Title Acta Medica Okayama
Volume volume66
Issue issue3
Publisher Okayama University Medical School
Start Page 239
End Page 244
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22729104
Web of Sience KeyUT 000305669700007
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/48881
JaLCDOI 10.18926/AMO/32110
FullText URL fulltext.pdf
Author Shinji, Toshiyuki| Kyaw, Yi Yi| Gokan, Katsunori| Tanaka, Yasuhito| Ochi, Koji| Kusano, Nobuchika| Mizushima, Takaaki| Fujioka, Shin-ichi| Shiraha, Hidenori| Lwin, Aye Aye| Shiratori, Yasushi| Mizokami, Masashi| Khin, Myo| Miyahara, Masayuki| Okada, Shigeru| Koide, Norio|
Abstract The prevalence of hepatitis C virus (HCV) genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR) amplified. Among them, 35 (31.8%) were of genotype 1, 52 (47.3%) were of genotype 3, and 23 (20.9%) were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.
Keywords hepatitis C virus(HCV)genotype type 6 variant Myanmar Southeast Asia phylogenetic analysis
Amo Type Article
Published Date 2004-06
Publication Title Acta Medica Okayama
Volume volume58
Issue issue3
Publisher Okayama University Medical School
Start Page 135
End Page 142
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 15471435
Web of Sience KeyUT 000222273300004
JaLCDOI 10.18926/AMO/31341
FullText URL fulltext.pdf
Author Mizushima, Takaaki| Ochi, Koji| Seno, Toshinobu| Matsumura, Naoki| Harada, Hideo|
Abstract We simultaneously assessed gastric emptying and gallbladder contraction after oral administration of a liquid meal by noninvasive ultrasonography in 17 patients with chronic pancreatitis (CP) and in 17 healthy controls. Gastrointestinal (GI) transit was also assessed by a noninvasive radioopaque marker method. Exocrine pancreatic function was evaluated by analyzing pure pancreatic juice and by analyzing the autonomic nervous system by cardiovascular reflex tests. Patients with CP showed impaired gallbladder contraction at 15 min and hastened gastric emptying. The cause of the former is unclear, whereas the latter was closely related with decreased pancreatic lipase output, but not with autonomic dysfunction. GI transit time did not differ between controls and patients with CP. In conclusion, we succeeded in clearly demonstrating impaired gallbladder contraction and hastened gastric emptying in patients with CP by a single noninvasive test, ultrasonography. We also revealed for the first time that hastened gastric emptying is associated with insufficient pancreatic lipase output.
Keywords chronic pancreatits gastric emptying gallbladder motility colonic transit lipase output
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 55
End Page 61
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548995
Web of Sience KeyUT 000072264100008
Author Ochi, Koji| Harada, Hideo| Matsumoto, Shuji| Seno, Tosinobu| Tanaka, Juntaro| Riaz Chowdhury| Mizushima, Takaaki| Sugimoto, Keisuke| Mitsunobu, Fumihiro| Tanizaki, Yoshiro| Nakai, Mutsuo| Akiyama, Tsuneo| Hasuoka, Hideaki|
Published Date 1993-06
Publication Title 岡大三朝分院研究報告
Volume volume64
Content Type Departmental Bulletin Paper
Author Chowdhury, Riaz| Ochi, Koji| Tanaka, Juntaro| Matsumoto, Shooji| Senou, Toshinobu| Harada, Hideo| Mizushima, Takaaki| Tanizaki, Yoshiro|
Published Date 1994-09
Publication Title 岡大三朝分院研究報告
Volume volume65
Content Type Departmental Bulletin Paper
Author Ochi, Koji| Harada, Hideo| Chowdhury, Riaz| Tanaka, Juntaro| Matsumoto, Shuji| Seno, Toshinobu| Mizushima, Takaaki| Mitsunobu, Fumihiro| Tanizaki, Yoshiro| Akiyama, Tsuneo| Nakai, Mutsuo| Hasuoka, Hideaki| Kato, Tadahiro|
Published Date 1994-09
Publication Title 岡大三朝分院研究報告
Volume volume65
Content Type Departmental Bulletin Paper
Author Chowdhury Riaz| Ochi, Koji| Harada, Hideo| Tanaka, Juntaro| Mizushima, Takaaki| Matsumoto, Shuji| Seno, Toshinobu| Ichimura, Mitsuko| Akiyama, Tsuneo| Yokota, Satoshi|
Published Date 1995-09
Publication Title 岡大三朝分院研究報告
Volume volume66
Content Type Departmental Bulletin Paper
Author Ochi, Koji| Harada, Hideo| Chowdhury Riaz| Yamashita, Haruhiro| Ichimura, Mitsuko| Tanaka, Juntaro| Seno, Toshinobu| Matsumoto, Shuji| Mizushima, Takaaki| Yokota, Satoshi| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 1995-09
Publication Title 岡大三朝分院研究報告
Volume volume66
Content Type Departmental Bulletin Paper
Author Yamamoto, Ryoichi| Ochi, Koji| Matsumura, Naoki| Tanaka, Juntaro| Kato, Tadahiro| Mizushima, Takaaki| Chowdhury Riaz| Harada, Hideo| Hasuoka, Hideaki| Yokota, Satoshi| Tanizaki, Yoshiro|
Published Date 1996-11
Publication Title 岡大三朝分院研究報告
Volume volume67
Content Type Departmental Bulletin Paper
Author Ochi, Koji| Chowdhury, Riaz| Ichimura, Mitsuko| Matsumura, Naoki| Tanaka, Juntaro| Mizushima, Takaaki| Yamamoto, Ryoichi| Harada, Hideo| Yokota, Satoshi| Mitsunobu, Fumihiro| Hosaki, Yasuhiro| Tanizaki, Yoshiro|
Published Date 1996-11
Publication Title 岡大三朝分院研究報告
Volume volume67
Content Type Departmental Bulletin Paper
Author Mizushima, Takaaki| Yamamoto, Ryoichi| Matsumura, Naoki| Chowdhury, Riaz| Kato, Tadahiro| Ochi, Koji| Tanaka, Juntaro| Harada, Hideo| Yokota, Satoshi| Mitsunobu, Fumihiro| Mifune, Takashi| Tanizaki, Yoshiro|
Published Date 1996-11
Publication Title 岡大三朝分院研究報告
Volume volume67
Content Type Departmental Bulletin Paper
Author Kato, Tadahiro| Hasuoka, Hideaki| Ochi, Koji| Mizushima, Takaaki| Chowdhury Riaz| Matsumura, Naoki| Yamamoto, Ryoichi| Tanaka, Juntaro| Harada, Hideo| Akiyama, Tsuneo| Yokota, Satoshi| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 1996-11
Publication Title 岡大三朝分院研究報告
Volume volume67
Content Type Departmental Bulletin Paper
Author Ochi, Koji| Matsumura, Naoki| Kato, Tadahiro| Mizushima, Takaaki| Ichimura, Mitsuko| Harada, Hideo| Tanizaki, Yoshiro| Mitsunobu, Fumihiro| Hasuoka, Hideaki| Tsurumi, Tetsuya|
Published Date 1997-12
Publication Title 岡大三朝分院研究報告
Volume volume68
Content Type Departmental Bulletin Paper
Author Mizushima, Takaaki| Ochi, Koji| Matsumura, Naoki| Kato, Tadahiro| Seno, Toshinobu| Harada, Hideo| Mifune, Takashi| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 1997-12
Publication Title 岡大三朝分院研究報告
Volume volume68
Content Type Departmental Bulletin Paper
Author Mizushima, Takaaki| Ochi, Koji| Matsumura, Naoki| Seno, Toshinobu| Harada, Hideo| Mifune, Takashi| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 1997-12
Publication Title 岡大三朝分院研究報告
Volume volume68
Content Type Departmental Bulletin Paper
Author Ochi, Koji| Matsumura, Naoki| Ichimura, Mitsuko| Mizushima, Takaaki| Harada, Hideo| Hasuoka, Hideaki| Yokoyama, Motohiro| Tsurumi, Tetsuya| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 1998-12
Publication Title 岡大三朝分院研究報告
Volume volume69
Content Type Departmental Bulletin Paper
Author Ochi, Koji| Matsumura, Naoki| Mizushima, Takaaki| Hasuoka, Hideaki| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Ochi, Koji| Matsumura, Naoki| Mizushima, Takaaki| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 1999-12
Publication Title 岡大三朝分院研究報告
Volume volume70
Content Type Departmental Bulletin Paper
Author Yokoyama, Motohiro| Ochi, Koji| Mizushima, Takaaki| Ichimura, Mitsuko| Tsurumi, Tetsuya| Mitsunobu, Fumihiro| Tanizaki, Yoshiro|
Published Date 2001-02-01
Publication Title 岡大三朝分院研究報告
Volume volume71
Content Type Departmental Bulletin Paper