ID | 31971 |
JaLCDOI | |
FullText URL | |
Author |
Imabayashi, Kiyomi
Inagaki, Sachiyo
Doi, Yusuke
Ishizu, Hideo
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Abstract | We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine. |
Keywords | HLA-DRB1 genotyping
group specific primer
single nucleotide polymorphism
multiplex primer extension reactions
application to mixed samples
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 2005-10
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Volume | volume59
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Issue | issue5
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Publisher | Okayama University Medical School
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Start Page | 179
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End Page | 194
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |