ID | 48853 |
FullText URL | |
Author |
Kojima, Toru
Hashimoto, Yuuri
Watanabe, Yuichi
Kuroda, Shinji
Kyo, Satoru
Mizuguchi, Hiroyuki
Urata, Yasuo
Tanaka, Noriaki
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Abstract | The presence of circulating tumor cells (CTCs) in the peripheral blood is associated with short survival, making the detection of CTCs clinically useful as a prognostic factor of disease outcome and/or a surrogate marker of treatment response. Recent technical advances in immunocytometric analysis and quantitative real-time PCR have made it possible to detect a few CTCs in the blood; however, there is no sensitive assay to specifically detect viable CTCs. Here, we report what we believe to be a new approach to visually detect live human CTCs among millions of peripheral blood leukocytes, using a telomerase-specific replication-selective adenovirus expressing GFP. First, we constructed a GFP-expressing attenuated adenovirus, in which the telomerase promoter regulates viral replication (OBP-401; TelomeScan). We then used OBP-401 to establish a simple ex vivo method that was able to detect viable human CTCs in the peripheral blood. The detection method involved a 3-step procedure, including the lysis of rbc, the subsequent addition of OBP-401 to the cell pellets, and an automated scan using fluorescence microscopy. OBP-401 infection increased the signal-to-background ratio as a tumor-specific probe, because the fluorescent signal was amplified only in viable, infected human tumor cells, by viral replication. This GFP-expressing virus-based method is remarkably simple and allows precise enumeration of CTCs.
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Published Date | 2009-10-01
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Publication Title |
The Journal of Clinical Investigation
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Volume | volume119
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Issue | issue10
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Publisher | American Society for Clinical Investigation
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Start Page | 3172
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End Page | 3181
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ISSN | 0021-9738
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NCID | AA00695520
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Content Type |
Journal Article
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Project |
Innovation Center Okayama for Nanobio-targeted Therapy(ICONT)
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Official Url | http://www.jci.org/articles/view/38609
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language |
English
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Copyright Holders | © 2009, American Society for Clinical Investigation
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File Version | publisher
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Refereed |
True
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DOI | |
PubMed ID | |
Web of Science KeyUT |