ID | 30764 |
JaLCDOI | |
FullText URL | |
Author |
Ino, Hideo
Hayashi, Kazuhiko
Teramoto, Norihiro
Koirala, Tirtha Raj
Chen, Hong-Li
Takahashi, Kiyoshi
Akagi, Tadaastu
|
Abstract | A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV. |
Keywords | Epstein-Barr virus
HVMF 1
lymphoma
?monkey cell line
PCR
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Amo Type | Article
|
Publication Title |
Acta Medica Okayama
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Published Date | 1997-08
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Volume | volume51
|
Issue | issue4
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Publisher | Okayama University Medical School
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Start Page | 207
|
End Page | 212
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |