ID | 49924 |
FullText URL | |
Author |
Shimoura, Yasumasa
Nakamura, Satoko
Mano, Shyouhe
Yoshino, Tadashi
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Abstract | Objective: We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis. Study Design: We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients. Results: On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma. Conclusion: IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.
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Keywords | Cytology
Immunocytochemical
double staining
Immunoglobulin light-chain restriction
Lymph node
B cell lymphoma
Flow cytometry
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Published Date | 2013
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Publication Title |
Acta Cytologica
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Volume | volume57
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Issue | issue1
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Start Page | 84
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End Page | 90
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ISSN | 0001-5547
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Content Type |
Journal Article
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Official Url | http://dx.doi.org/10.1159/000342475
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Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/49730
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language |
English
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Copyright Holders | Copyright 2012 S. Karger AG, Basel
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File Version | author
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Refereed |
True
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DOI | |
Web of Science KeyUT |