ID | 31091 |
JaLCDOI | |
FullText URL | |
Author |
Teramoto, Norihiro
Tonoyama, Yuji
Akagi, Tadaatsu
Sarker, Ashit Baran
Yamadori, Ichiro
Takahashi, Kiyoshi
|
Abstract | The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells. |
Keywords | polymerase chain reaction
human T-lymphotropic virus type I
paraffin section
single cell
single copy gene
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Amo Type | Article
|
Publication Title |
Acta Medica Okayama
|
Published Date | 1994-08
|
Volume | volume48
|
Issue | issue4
|
Publisher | Okayama University Medical School
|
Start Page | 189
|
End Page | 193
|
ISSN | 0386-300X
|
NCID | AA00508441
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Content Type |
Journal Article
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language |
English
|
File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |