FullText URL | fulltext.pdf |
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Author | Tang, Shaoying| Yonezawa, Tomoko| Maeda, Yukihide| Ono, Mitsuaki| Maeba, Takahiro| Miyoshi, Toru| Momota, Ryusuke| Tomono, Yasuko| Oohashi, Toshitaka| |
Published Date | 2021-04-13 |
Publication Title | PLoS ONE |
Volume | volume16 |
Issue | issue4 |
Publisher | Public Library Science |
Start Page | e0249909 |
ISSN | 1932-6203 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2021 Tang et al. |
File Version | publisher |
PubMed ID | 33848312 |
NAID | 120007042400 |
DOI | 10.1371/journal.pone.0249909 |
Web of Science KeyUT | 000640373500028 |
Related Url | isVersionOf https://doi.org/10.1371/journal.pone.0249909 |
Title Alternative | Practical data science with R/Python for beginners |
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FullText URL | 132_148.pdf |
Author | Momota, Ryusuke| |
Keywords | データサイエンス MOOCs R Python Jupyter notebook |
Publication Title | Journal of Okayama Medical Association |
Published Date | 2020-12-01 |
Volume | volume132 |
Issue | issue3 |
Start Page | 148 |
End Page | 151 |
ISSN | 0030-1558 |
Related Url | isVersionOf https://doi.org/10.4044/joma.132.148 |
language | Japanese |
Copyright Holders | Copyright (c) 2020 岡山医学会 |
File Version | publisher |
DOI | 10.4044/joma.132.148 |
NAID | 130007950569 |
Author | Shinaoka, Akira| Momota, Ryusuke| Shiratsuchi, Eri| Kosaka, Mitsuko| Kumagishi, Kanae| Nakahara, Ryuichi| Naito, Ichiro| Ohtsuka, Aiji| |
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Published Date | 2013-04 |
Publication Title | Microscopy and Microanalysis |
Volume | volume19 |
Issue | issue2 |
Content Type | Journal Article |
Author | Momota, Ryusuke| Naito, Ichiro| Ninomiya, Yoshifumi| Ohtsuka, Aiji| |
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Published Date | 2011-05 |
Publication Title | Matrix Biology |
Volume | volume30 |
Issue | issue4 |
Content Type | Journal Article |
JaLCDOI | 10.18926/AMO/32859 |
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FullText URL | fulltext.pdf |
Author | Murakami, Shinichiro| Fujino, Hidemi| Takeda, Isao| Momota, Ryusuke| Kumagishi, Kanae| Ohtsuka, Aiji| |
Abstract | The skeletal muscle is classified into 2 types, slow oxidative or fast glycolytic muscle. For further characterization, we investigated the capillary architecture in slow and fast muscles. The rat soleus and extensor digitorum longus (EDL) muscles were used as representatives of slow and fast muscles, respectively. To investigate capillary density, sections of both types of muscle were stained with alkaline phosphatase;the soleus muscle showed more intense reactivity, indicating that it had a denser capillary structure than the EDL muscle. We then injected fluorescent contrast medium into samples of both muscle types for light and confocal-laser microscopic evaluation. The capillary density and capillary-to-fiber ratio were significantly higher, and the course of the capillaries was more tortuous, in the soleus muscle than in the EDL muscle. Capillary coursed more tortuously in the soleus than in the EDL muscle. Succinate dehydrogenase (SDH) activity, an indicator of mitochondrial oxidative capacity, and vascular endothelial growth factor (VEGF) expression were also significantly higher in the soleus muscle. Thus, we conclude that slow oxidative muscle possess a rich capillary structure to provide demanded oxygen, and VEGF might be involved in the formation and/or maintenance of this highly capillarized architecture. |
Keywords | skeletal muscle capillaly succinate dehydrogenase activity vascular endothelial growth factor |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2010-02 |
Volume | volume64 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 11 |
End Page | 18 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 20200579 |
Web of Science KeyUT | 000274868300002 |
JaLCDOI | 10.18926/AMO/30753 |
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FullText URL | fulltext.pdf |
Author | Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji| |
Abstract | We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy. |
Keywords | colloidal iron microwave histochemistry transmission electron microscopy |
Amo Type | Article |
Publication Title | Acta Medica Okayama |
Published Date | 2006-02 |
Volume | volume60 |
Issue | issue1 |
Publisher | Okayama University Medical School |
Start Page | 59 |
End Page | 64 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 16508690 |
Web of Science KeyUT | 000235538900007 |
Author | 百田 龍輔| |
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Published Date | 2001-03-25 |
Publication Title | |
Content Type | Thesis or Dissertation |