ID | 63931 |
FullText URL | |
Author |
Okada, Satoshi
Group of Environmental Stress Response Systems, Institute of Plant Science and Resources, Okayama University
Lei, Gui J.
Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
Yamaji, Naoki
Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
Huang, Sheng
Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
Ma, Jian F.
Group of Plant Stress Physiology, Institute of Plant Science and Resources, Okayama University
Mochida, Keiichi
Crop Design Research Team, Institute of Plant Science and Resources, Okayama University
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Hirayama, Takashi
Group of Environmental Stress Response Systems, Institute of Plant Science and Resources, Okayama University
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Abstract | FE UPTAKE-INDUCING PEPTIDE1 (FEP1), also named IRON MAN3 (IMA3) is a short peptide involved in the iron deficiency response in Arabidopsis thaliana. Recent studies uncovered its molecular function, but its physiological function in the systemic Fe response is not fully understood. To explore the physiological function of FEP1 in iron homoeostasis, we performed a transcriptome analysis using the FEP1 loss-of-function mutant fep1-1 and a transgenic line with oestrogen-inducible expression of FEP1. We determined that FEP1 specifically regulates several iron deficiency-responsive genes, indicating that FEP1 participates in iron translocation rather than iron uptake in roots. The iron concentration in xylem sap under iron-deficient conditions was lower in the fep1-1 mutant and higher in FEP1-induced transgenic plants compared with the wild type (WT). Perls staining revealed a greater accumulation of iron in the cortex of fep1-1 roots than in the WT root cortex, although total iron levels in roots were comparable in the two genotypes. Moreover, the fep1-1 mutation partially suppressed the iron overaccumulation phenotype in the leaves of the oligopeptide transporter3-2 (opt3-2) mutant. These data suggest that FEP1 plays a pivotal role in iron movement and in maintaining the iron quota in vascular tissues in Arabidopsis.
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Keywords | oestrogen induction system
fep1-1
iron-deficiency response
transcriptome
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Note | This is the peer reviewed version of the following article: [Okada, S., Lei, G.J., Yamaji, N., Huang, S., Ma, J.F., Mochida, K., et al. (2022) FE UPTAKE-INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of Arabidopsis. Plant, Cell & Environment, 45, 3322–3337. https://doi.org/10.1111/pce.14424], which has been published in final form at [https://doi.org/10.1111/pce.14424]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages there of by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
This fulltext is available in Sep. 2023. |
Published Date | 2022-09-07
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Publication Title |
Plant, Cell and Environment
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Volume | volume45
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Issue | issue11
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Publisher | Wiley
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Start Page | 3322
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End Page | 3337
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ISSN | 0140-7791
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NCID | AA0036238X
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022 John Wiley & Sons Ltd.
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File Version | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1111/pce.14424
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Citation | Okada, S., Lei, G.J., Yamaji, N., Huang, S., Ma, J.F., Mochida, K., et al. (2022) FE UPTAKE-INDUCING PEPTIDE1 maintains Fe translocation by controlling Fe deficiency response genes in the vascular tissue of Arabidopsis. Plant, Cell & Environment, 45, 3322–3337. https://doi.org/10.1111/pce.14424
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
Japan Science and Technology Agency
Bio-oriented Technology Research Advancement Institution
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助成番号 | 19K22434
21H02509
16H06296
21H05034
JPMJCR16O4
JPJ009237
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