ID | 64316 |
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Author |
Wu, Hongyan
School of Food Science and Technology, Dalian Polytechnic University
Nakamura, Toshiyuki
Graduate School of Environmental and Life Science, Okayama University
Guo, Yingnan
School of Food Science and Technology, Dalian Polytechnic University
Matsumoto, Riho
Graduate School of Environmental and Life Science, Okayama University
Munemasa, Shintaro
Graduate School of Environmental and Life Science, Okayama University
ORCID
Murata, Yoshiyuki
Graduate School of Environmental and Life Science, Okayama University
ORCID
Kaken ID
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Nakamura, Yoshimasa
Graduate School of Environmental and Life Science, Okayama University
ORCID
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Abstract | Since brown rice extract is a rich source of biologically active compounds, the present study is aimed to quantify the major compounds in brown rice and to compare their cytoprotective potential against oxidative stress. The content of the main hydrophobic compounds in brown rice followed the order of cycloartenyl ferulate (CAF) (89.00 +/- 8.07 nmol/g) >> alpha-tocopherol (alpha T) (19.73 +/- 2.28 nmol/g) > gamma-tocotrienol (gamma T3) (18.24 +/- 1.41 nmol/g) > alpha-tocotrienol (alpha T3) (16.02 +/- 1.29 nmol/g) > gamma-tocopherol (gamma T) (3.81 +/- 0.40 nmol/g). However, the percent contribution of CAF to the radical scavenging activity of one gram of whole brown rice was similar to those of alpha T, alpha T3, and gamma T3 because of its weaker antioxidant activity. The CAF pretreatment displayed a significant cytoprotective effect on the hydrogen peroxide-induced cytotoxicity from 10 mu M, which is lower than the minimal concentrations of alpha T and gamma T required for a significant protection. CAF also enhanced the nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation coincided with the enhancement of the heme oxygenase-1 (HO-1) mRNA level. An HO-1 inhibitor, tin protoporphyrin IX (SnPP), significantly impaired the cytoprotection of CAF. The cytoprotective potential of CAF is attributable to its cycloartenyl moiety besides the ferulyl moiety. These results suggested that CAF is the predominant cytoprotector in brown rice against hydrogen peroxide-induced cytotoxicity.
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Keywords | cycloartenyl ferulate
antioxidative effect
cytoprotective potential
heme oxygenase-1
nuclear factor erythroid 2-related factor 2
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Published Date | 2023-01-03
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Publication Title |
International Journal Of Molecular Sciences
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Volume | volume24
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Issue | issue1
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Publisher | MDPI
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Start Page | 822
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ISSN | 1422-0067
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2023 by the authors.
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File Version | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.3390/ijms24010822
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License | https://creativecommons.org/licenses/by/4.0/
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | 17H04725
21K11676
16K14928
17H03818
20H02933
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