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Ono-Minagi, Hitomi Department of Cytology and Histology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine
Nohno, Tsutomu Department of Cytology and Histology, Okayama University Medical School
Takabatake, Kiyofumi Department of Oral Pathology and Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Tanaka, Takehiro Department of Pathology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons
Katsuyama, Takayuki Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Hospital
Miyawaki, Kohta Division of Precision Medicine, Kyushu University School of Medicine
Wada, Jun Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Ibaragi, Soichiro Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Iida, Seiji Department of Oral and Maxillofacial Reconstructive Surgery, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Yoshino, Tadashi Department of Pathology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine Kaken ID publons researchmap
Nagatsuka, Hitoshi Department of Oral Pathology and Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Sakai, Takayoshi Department of Rehabilitation for Orofacial Disorders, Osaka University Graduate School of Dentistry
Ohuchi, Hideyo Department of Cytology and Histology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID researchmap
Abstract
Some forms of Sjögren’s syndrome (SS) follow a clinical course accompanied by systemic symptoms caused by lymphocyte infiltration and proliferation in the liver, kidneys, and other organs. To better understand the clinical outcomes of SS, here we used minor salivary gland tissues from patients and examine their molecular, biological, and pathological characteristics. A retrospective study was performed, combining clinical data and formalin-fixed paraffin-embedded (FFPE) samples from female patients over 60 years of age who underwent biopsies at Okayama University Hospital. We employed direct digital RNA counting with nCounter® and multiplex immunofluorescence analysis with a PhenoCycler™ on the labial gland biopsies. We compared FFPE samples from SS patients who presented with other connective tissue diseases (secondary SS) with those from stable SS patients with symptoms restricted to the exocrine glands (primary SS). Secondary SS tissues showed enhanced epithelial damage and lymphocytic infiltration accompanied by elevated expression of autophagy marker genes in the immune cells of the labial glands. The close intercellular distance between helper T cells and B cells positive for autophagy-associated molecules suggests accelerated autophagy in these lymphocytes and potential B cell activation by helper T cells. These findings indicate that examination of FFPE samples from labial gland biopsies can be an effective tool for evaluating molecular histological differences between secondary and primary SS through multiplexed analysis of gene expression and tissue imaging.
Keywords
Autoimmune disease
Xerostomia
Multiplex immunostaining
Spatial analysis
Autophagy
Note
The version of record of this article, first published in BMC Oral Health, is available online at Publisher’s website: http://dx.doi.org/10.1186/s12903-024-04869-4
Published Date
2024-09-16
Publication Title
BMC Oral Health
Volume
volume24
Issue
issue1
Publisher
BMC
Start Page
1099
ISSN
1472-6831
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2024.
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DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1186/s12903-024-04869-4
License
http://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Ono-Minagi, H., Nohno, T., Takabatake, K. et al. Histological differences related to autophagy in the minor salivary gland between primary and secondary types of Sjögren’s syndrome. BMC Oral Health 24, 1099 (2024). https://doi.org/10.1186/s12903-024-04869-4
Funder Name
Japan Society for the Promotion of Science
助成番号
JP21K10093
JP19KK0230
JP 22KJ230305