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Otani, Yusuke Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yoshikawa, Soichiro Department of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID researchmap
Nagao, Kei Department of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Tanaka, Takehiro Department of Pathology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID publons
Toyooka, Shinichi Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID publons researchmap
Fujimura, Atsushi Department of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID researchmap
Abstract
Mast cells are present in mucosal and connective tissues throughout the body. They synthesize and release a wide variety of bioactive molecules, such as histamine, proteases, and cytokines. In this study, we found that a population of connective tissue mast cells (CTMCs) stores and releases noradrenaline, originating from sympathetic nerves. Noradrenaline-storing cells, not neuronal fibers, were predominantly identified in the connective tissues of the skin, mammary gland, gastrointestinal tract, bronchus, thymus, and pancreas in wild-type mice but were absent in mast cell-deficient W-sash c-kit mutant KitW-sh/W-sh mice. In vitro studies using bone marrow-derived mast cells revealed that extracellular noradrenaline was taken up but not synthesized. Upon ionomycin stimulation, noradrenaline was released. Electron microscopy analyses further suggested that noradrenaline is stored in and released from the secretory granules of mast cells. Finally, we found that noradrenaline-storing CTMCs express organic cation transporter 3 (Oct3), which is also known as an extraneuronal monoamine transporter, SLC22A3. Our findings indicate that mast cells may play a role in regulating noradrenaline concentration by storing and releasing it in somatic tissues.
Keywords
Mast cells
Connective tissue mast cells
Noradrenaline
Immunoelectron microscopy
SLC22A3
Note
The version of record of this article, first published in The Journal of Physiological Sciences, is available online at Publisher’s website: http://dx.doi.org/10.1186/s12576-023-00883-3
Published Date
2023-10-12
Publication Title
The Journal of Physiological Sciences
Volume
volume73
Issue
issue1
Publisher
BMC
Start Page
24
ISSN
1880-6546
NCID
AA12129145
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2023.
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DOI
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isVersionOf https://doi.org/10.1186/s12576-023-00883-3
License
http://creativecommons.org/licenses/by/4.0/
Citation
Otani, Y., Yoshikawa, S., Nagao, K. et al. Connective tissue mast cells store and release noradrenaline. J Physiol Sci 73, 24 (2023). https://doi.org/10.1186/s12576-023-00883-3
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development
助成番号
JP22K09004
JP23K06676
JP18cm0106143
JP20cm0106179