ID | 63959 |
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Author |
Ushio, Soichiro
Department of Pharmacy, Okayama University Hospital
Wada, Yudai
Department of Clinical Pharmacy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nakamura, Mizuki
Department of Clinical Pharmacy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Matsumoto, Daiki
Department of Clinical Pharmacy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Hoshika, Kota
Department of Clinical Pharmacy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Shiromizu, Shoya
Department of Pharmacy, Okayama University Hospital
Iwata, Naohiro
Department of Pharmacy, Okayama University Hospital
Esumi, Satoru
Department of Pharmacy, Okayama University Hospital
Kajizono, Makoto
Department of Pharmacy, Okayama University Hospital
Kitamura, Yoshihisa
Department of Pharmacy, Okayama University Hospital
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Abstract | Hochuekkito (HET) is a Kampo medicine used to treat postoperative and post-illness general malaise and decreased motivation. HET is known to regulate immunity and modulate inflammation. However, the precise mechanism and effects of HET on inflammation-induced central nervous system disorders remain unclear. This study aimed to assess the effect of HET on inflammation-induced anxiety-like behavior and the mechanism underlying anxiety-like behavior induced by lipopolysaccharide (LPS). Institute of Cancer Research mice were treated with LPS (300 mu g/kg, intraperitoneally), a bacterial endotoxin, to induce systemic inflammation. The mice were administered HET (1.0 g/kg, orally) once a day for 2 weeks before LPS treatment. The light-dark box test and the hole-board test were performed 24 h after the LPS injection to evaluate the effects of HET on anxiety-like behaviors. Serum samples were obtained at 2, 5, and 24 h after LPS injection, and interleukin-6 (IL-6) levels in serum were measured. Human and mouse macrophage cells (THP-1 and RAW264.7 cells, respectively) were used to investigate the effect of HET on LPS-induced IL-6 secretion. The repeated administration of HET prevented anxiety-like behavior and decreased serum IL-6 levels in LPS-treated mice. HET significantly suppressed LPS-induced IL-6 secretion in RAW264.7 and THP-1 cells. Similarly, glycyrrhizin, one of the chemical constituents of HET, suppressed LPS-induced anxiety-like behaviors. Our study revealed that HET ameliorated LPS-induced anxiety-like behavior and inhibited IL-6 release in vivo and in vitro. Therefore, we postulate that HET may be useful against inflammation-induced anxiety-like behavior.
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Keywords | anxiolytic
inflammation
immunomodulation
macrophages
Kampo medicine
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Published Date | 2022-08-12
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Publication Title |
Frontiers In Pharmacology
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Volume | volume13
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Publisher | Frontiers Media S.A.
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Start Page | 890048
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ISSN | 1663-9812
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022 Ushio, Wada, Nakamura, Matsumoto, Hoshika, Shiromizu, Iwata, Esumi, Kajizono, Kitamura and Sendo.
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.3389/fphar.2022.890048
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License | https://creativecommons.org/licenses/by/4.0/
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