| Author | Tanaka, Shigetomi| Shiraha, Hidenori| Nakanishi, Yutaka| Nishina, Shin-Ichi| Matsubara, Minoru| Horiguchi, Shigeru| Takaoka, Nobuyuki| Iwamuro, Masaya| Kataoka, Junro| Kuwaki, Kenji| Hagihara, Hiroaki| Toshimori, Junichi| Ohnishi, Hideki| Takaki, Akinobu| Nakamura, Shinichiro| Nouso, Kazuhiro| Yagi, Takahito| Yamamoto, Kazuhide| |
|---|---|
| Published Date | 2012-12-01 |
| Publication Title | International Journal of Cancer |
| Volume | volume131 |
| Issue | issue11 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/49042 |
|---|---|
| FullText URL | 66_6_461.pdf |
| Author | Koike, Kazuko| Takaki, Akinobu| Kato, Nobuyuki| Ouchida, Mamoru| Kanzaki, Hirotaka| Yasunaka, Tetsuya| Shiraha, Hidenori| Miyake, Yasuhiro| Yamamoto, Kazuhide| |
| Abstract | Hepatitis C virus (HCV) infection induces several changes in hepatocytes, such as oxidative stress, steatosis, and hepatocarcinogenesis. Although considerable progress has been made during recent years, the mechanisms underlying these functions remain unclear. We employed proteomic techniques in HCV replicon-harboring cells to determine the effects of HCV replication on host-cell protein expression. We examined two-dimensional electrophoresis (2-DE) and mass spectrometry to compare and identify differentially expressed proteins between HCV subgenomic replicon-harboring cells and their “cured” cells. One of the identified proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Full-length HCV genome RNA replicating and cured cells were also assessed using ELISA. Replicon-harboring cells showed higher expression of retinal dehydrogenase 1 (RALDH-1), which converts retinol to retinoic acid, and the cured cells showed higher expression of retinol-binding protein (RBP), which transports retinol from the liver to target tissues. The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression. |
| Keywords | hepatitis C virus retinol-binding protein |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2012-12 |
| Volume | volume66 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 461 |
| End Page | 468 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2012 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23254580 |
| Web of Science KeyUT | 000312966100005 |
| Author | Nakanishi, Yutaka| Shiraha, Hidenori| Nishina, Shin-ichi| Tanaka, Shigetomi| Matsubara, Minoru| Horiguchi, Shigeru| Iwamuro, Masaya| Takaoka, Nobuyuki| Uemura, Masayuki| Kuwaki, Kenji| Hagihara, Hiroaki| Toshimori, Junichi| Ohnishi, Hideki| Takaki, Akinobu| Nakamura, Shinichiro| Kobayashi, Yoshiyuki| Nouso, Kazuhiro| Yagi, Takahito| Yamamoto, Kazuhide| |
|---|---|
| Published Date | 2011-01-04 |
| Publication Title | BMC Cancer |
| Volume | volume11 |
| Content Type | Journal Article |
| Author | Matsuo, Noriyuki| Shiraha, Hidenori| Fujikawa, Tatsuya| Takaoka, Nobuyuki| Ueda, Naoki| Tanaka, Shigetomi| Nishina, Shinichi| Nakanishi, Yutaka| Uemura, Masayuki| Takaki, Akinobu| Nakamura, Shinichiro| Kobayashi, Yoshiyuki| Nouso, Kazuhiro| Yagi, Takahito| Yamamoto, Kazuhide| |
|---|---|
| Published Date | 2009-07-18 |
| Publication Title | BMC Cancer |
| Volume | volume9 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/AMO/32110 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Shinji, Toshiyuki| Kyaw, Yi Yi| Gokan, Katsunori| Tanaka, Yasuhito| Ochi, Koji| Kusano, Nobuchika| Mizushima, Takaaki| Fujioka, Shin-ichi| Shiraha, Hidenori| Lwin, Aye Aye| Shiratori, Yasushi| Mizokami, Masashi| Khin, Myo| Miyahara, Masayuki| Okada, Shigeru| Koide, Norio| |
| Abstract | The prevalence of hepatitis C virus (HCV) genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR) amplified. Among them, 35 (31.8%) were of genotype 1, 52 (47.3%) were of genotype 3, and 23 (20.9%) were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed. |
| Keywords | hepatitis C virus(HCV)genotype type 6 variant Myanmar Southeast Asia phylogenetic analysis |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2004-06 |
| Volume | volume58 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 135 |
| End Page | 142 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 15471435 |
| Web of Science KeyUT | 000222273300004 |
| JaLCDOI | 10.18926/AMO/31826 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Fujikawa, Tatsuya| Shiraha, Hidenori| Yamamoto, Kazuhide| |
| Abstract | Serum des-gamma-carboxy prothrombin (DCP) is commonly used to detect hepatocellular carcinoma (HCC). This review focuses on the clinical features of DCP-positive HCC and the molecular function of DCP in HCC. DCP-positive HCC demonstrates more aggressive clinicopathological features than DCP-negative HCC. Analysis of the biological effects of DCP revealed that DCP acts as a growth factor in both an autocrine and paracrine manner. DCP stimulates HCC cell proliferation through the Met-Janus kinase 1-signal transducer and activator of transcription 3 signaling pathway, whereas for vascular endothelial cells, it stimulates cell proliferation and migration through the kinase insert domain receptor-phospholipase C-gamma-mitogen-activated protein kinase signaling pathway. |
| Keywords | des-gamma-carboxy prothrombin hepatocellular carcinoma signaling pathway cell proliferation angiogenesis |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2009-12 |
| Volume | volume63 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 299 |
| End Page | 304 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 20035286 |
| Web of Science KeyUT | 000273145900001 |
| JaLCDOI | 10.18926/AMO/30405 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Tamura, Tomoyuki| Koide, Norio| Hada, Hajime| Shiraha, Hidenori| Tsuji, Takao| |
| Abstract | Adult rat hepatocytes assemble to form multicellular spheroids under non-adherent environments such as immobilized chondroitin sulfate-proteoglycan in primary culture. Previously, we demonstrated that hepatocyte spheroids exhibited various differentiated structures as observed in the liver tissue. It was also shown that hepatocyte growth was highly suppressed and several differentiated functions, including albumin production and gluconeogenesis, were well preserved in spheroids. To investigate the differentiated functions of cultured hepatocytes in relation to cell morphology, we compared the expression of the albumin and transferrin genes in spheroids with those in monolayers by Northern blot analysis. Production of these proteins in the culture medium was simultaneously examined by ELISA. Gene expression and protein production of both albumin and transferrin were better preserved in spheroids. We also examined changes in the expression of liver-specific genes in response to IL-6. Reduced mRNA levels of both albumin and transferrin was only found in spheroids and no change was observed in monolayers. These results suggest that the regulation of tissue-specific gene expression is better preserved in spheroids, in which hepatocytes are in close contact with each other. |
| Keywords | hepatocyte spheroid primary culture gene expression IL-6 |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 1995-06 |
| Volume | volume49 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 161 |
| End Page | 167 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 7676847 |
| Web of Science KeyUT | A1995RH05400007 |
| Author | Hanafusa, Tadashi| Shinji, Toshiyuki| Shiraha, Hidenori| Nouso, Kazuhiro| Iwasaki, Yoshiaki| Yumoto, Eichiro| Ono, Toshiro| Koide, Norio| |
|---|---|
| Published Date | 2005-01-20 |
| Publication Title | BMC Cancer |
| Volume | volume5 |
| Content Type | Journal Article |
| Author | Kobayashi, Yoshiyuki| Nakamura, Shinichiro| Ohnishi, Hideki| Toshimori, Junichi| Kuwaki, Kenji| Hagihara, Hiroaki| Miyake, Yasuhiro| Shiraha, Hidenori| Nouso, Kazuhiro| Yagi, Takahito| Tanaka, Noriaki| Yamamoto, Kazuhide| |
|---|---|
| Published Date | 2009-04-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume121 |
| Issue | issue1 |
| Content Type | Journal Article |
| Author | Fujikawa, Tatsuya| Shiraha, Hidenori| Yamamoto, Kazuhide| |
|---|---|
| Published Date | 2009-04-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume121 |
| Issue | issue1 |
| Content Type | Journal Article |
| Author | 白羽 英則| |
|---|---|
| Published Date | 1996-03-31 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
