ID | 63211 |
FullText URL | |
Author |
Kawashima, Takeshi
National Institute of Genetics
Yoshida, Masa-aki
Marine Biological Science Section, Education and Research Center Biological Resources, Faculty of Life and Environmental Science, Shimane University
Miyazawa, Hideyuki
National Institute of Genetics
Nakano, Hiroaki
Shimoda Marine Research Center, University of Tsukuba
Nakano, Natumi
Department of Biology, Nara Medical University
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Abstract | The dynamics of microscopic marine plankton in coastal areas is a fundamental theme in marine biodiversity research, but studies have been limited because the only available methodology was collection of plankton using plankton-nets and microscopic observation. In recent years, environmental DNA (eDNA) analysis has exhibited potential for conducting comprehensive surveys of marine plankton diversity in water at fixed points and depths in the ocean. However, few studies have examined how eDNA analysis reflects the actual distribution and dynamics of organisms in the field, and further investigation is needed to determine whether it can detect distinct differences in plankton density in the field. To address this, we analyzed eDNA in seawater samples collected at 1 km intervals at three depths over a linear distance of approximately 3.0 km in the Seto Inland Sea. The survey area included a location with a high density of Acoela (Praesagittifera naikaiensis). However, the eDNA signal for this was little to none, and its presence would not have been noticed if we did not have this information beforehand. Meanwhile, eDNA analysis enabled us to confirm the presence of a species of Placozoa that was previously undiscovered in the area. In summary, our results suggest that the number of sequence reads generated from eDNA samples in our project was not sufficient to predict the density of a particular species. However, eDNA can be useful for detecting organisms that have been overlooked using other methods.
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Keywords | eDNA
marine invertebrate
Xenacoelomorpha
Acoela
Praesagittifera naikaiensis
Placozoa
Trichoplax adhaerens
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Note | This fulltext is published by Zoological Society of Japan. To access the final published work, see https://doi.org/10.2108/zs210073 .
This full-text file will be available in Feb. 2023.
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Published Date | 2022-01-28
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Publication Title |
Zoological Science
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Volume | volume39
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Issue | issue1
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Publisher | Zoological Society of Japan
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Start Page | 157
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End Page | 165
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ISSN | 0289-0003
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NCID | AA10545874
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2022 Zoological Society of Japan
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File Version | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.2108/zs210073
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Funder Name |
Challenging Exploratory Research Project for the Future grant from the Research Organization of Information and Systems (ROIS)
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