ID | 67024 |
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Author |
Sakamoto, Hirotaka
Department of Biology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University
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Inutsuka, Ayumu
Division of Brain and Neurophysiology, Department of Physiology, Jichi Medical University
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Abstract | Recent advances in viral vector technology, specifically using adeno-associated virus (AAV) vectors, have significantly expanded possibilities in neuronal tracing. We have utilized the Cre/loxP system in combination with AAV techniques in rats to explore the subcellular localization of palmitoylation signal-tagged GFP (palGFP) in oxytocin-producing neurosecretory neurons. A distinctive branching pattern of single axons was observed at the level of the terminals in the posterior pituitary. Despite challenges in detecting palGFP signals by fluorescent microscopy, immunoelectron microscopy demonstrated predominant localization on the plasma membrane, with a minor presence on the neurosecretory vesicle membrane. These findings suggest that membrane-anchored palGFP may undergo exocytosis, translocating from the plasma membrane to the neurosecretory vesicle membrane. In this study, we observed characteristic axon terminal structures in the posterior pituitary of oxytocin neurons. This study indicates the importance of understanding the plasma membrane-specific sorting system in neuronal membrane migration and encourages future studies on the underlying mechanisms.
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Keywords | GFP with a palmitoylation signal (palGFP)
plasmalemma localization
neurosecretory vesicle
immunoelectron microscopy
oxytocin
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Published Date | 2024-04-25
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Publication Title |
ACTA HISTOCHEMICA ET CYTOCHEMICA
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Volume | volume57
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Issue | issue2
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Publisher | The Japan Society of Histochemistry and Cytochemistry
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Start Page | 85
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End Page | 88
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ISSN | 0044-5991
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NCID | AA00508022
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2024 The Japan Society of Histochemistry and Cytochemistry
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1267/ahc.24-00001
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License | https://creativecommons.org/licenses/by-nc/4.0/
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Funder Name |
Japan Society for the Promotion of Science
Takeda Science Foundation
Naito Foundation
Ryobi Teien Memory Foundation
Wesco Scientific Promotion Foundation
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助成番号 | 22H02656
22K19332
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