ID | 61324 |
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Author |
Otubo, Akito
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Kawakami, Natsuko
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Maejima, Sho
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Ueda, Yasumasa
Department of Physiology, Kyoto Prefectural University of Medicine
Morris, John F.
Department of Physiology, Anatomy & Genetics, University of Oxford
Sakamoto, Tatsuya
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
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Sakamoto, Hirotaka
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
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Abstract | Arginine vasopressin (AVP), when released into portal capillaries with corticotrophin‐releasing factor (CRF) from terminals of parvocellular neurones of the hypothalamic paraventricular nucleus (PVH), facilitates the secretion of adrenocorticotrophic hormone (ACTH) in stressed rodents. The AVP gene encodes a propeptide precursor containing AVP, AVP‐associated neurophysin II (NPII), and a glycopeptide copeptin, although it is currently unclear whether copeptin is always cleaved from the neurophysin and whether the NPII and/or copeptin have any functional role in the pituitary. Furthermore, for primates, it is unknown whether CRF, AVP, NPII and copeptin are all colocalised in neurosecretory vesicles in the terminal region of the paraventricular CRF neurone axons. Therefore, we investigated, by fluorescence and immunogold immunocytochemistry, the cellular and subcellular relationships of these peptides in the CRF‐ and AVP‐producing cells in unstressed Japanese macaque monkeys (Macaca fuscata). Reverse transcription‐polymerase chain reaction analysis showed the expression of both CRF and AVP mRNAs in the monkey PVH. As expected, in the magnocellular neurones of the PVH and supraoptic nucleus, essentially no CRF immunoreactivity could be detected in NPII‐immunoreactive (AVP‐producing) neurones. Immunofluorescence showed that, in the parvocellular part of the PVH, NPII was detectable in a subpopulation (approximately 39%) of the numerous CRF‐immunoreactive neuronal perikarya, whereas, in the outer median eminence, NPII was more prominent (approximately 52%) in the CRF varicosities. Triple immunoelectron microscopy in the median eminence demonstrated the presence of both NPII and copeptin immunoreactivity in dense‐cored vesicles of CRF‐containing axons. The results are consistent with an idea that the AVP propeptide is processed and NPII and copeptin are colocalised in hypothalamic‐pituitary CRF axons in the median eminence of a primate. The CRF, AVP and copeptin are all co‐packaged in neurosecretory vesicles in monkeys and are thus likely to be co‐released into the portal capillary blood to amplify ACTH release from the primate anterior pituitary.
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Keywords | corticotrophin‐releasing factor
Japanese macaque monkey (Macaca fuscata)
median eminence
paraventricular nucleus of the hypothalamus
vasopressin
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Published Date | 2020-05-23
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Publication Title |
Journal of Neuroendocrinology
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Volume | volume32
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Issue | issue8
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Publisher | Wiley
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Start Page | e12875
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ISSN | 0953-8194
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NCID | AA10755083
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2020 The Authors.
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File Version | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.1111/jne.12875
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License | https://creativecommons.org/licenses/by/4.0/
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Funder Name |
Japan Society for the Promotion of Science
Japan Agency for Medical Research and Development
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助成番号 | 15K15202
15KK0257
15H05724
16H06280
961149
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