ID | 60771 |
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Author |
Kawakami, Natsuko
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Otubo, Akito
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Maejima, Sho
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Talukder, Ashraf H.
Laboratory of Information Biology, Graduate School of Information Sciences, Tohoku University
Satoh, Keita
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Oti, Takumi
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Takanami, Keiko
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Ueda, Yasumasa
Department of Physiology, Kyoto Prefectural University of Medicine
Itoi, Keiichi
Laboratory of Information Biology, Graduate School of Information Sciences, Tohoku University
Morris, John F.
Department of Physiology, Anatomy and Genetics, University of Oxford
Sakamoto, Tatsuya
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
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Sakamoto, Hirotaka
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
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Abstract | Arginine vasopressin (AVP) is synthesized in parvocellular‐ and magnocellular neuroendocrine neurons in the paraventricular nucleus (PVN) of the hypothalamus. Whereas magnocellular AVP neurons project primarily to the posterior pituitary, parvocellular AVP neurons project to the median eminence (ME) and to extrahypothalamic areas. The AVP gene encodes pre‐pro‐AVP that comprises the signal peptide, AVP, neurophysin (NPII), and a copeptin glycopeptide. In the present study, we used an N‐terminal copeptin antiserum to examine copeptin expression in magnocellular and parvocellular neurons in the hypothalamus in the mouse, rat, and macaque monkey. Although magnocellular NPII‐expressing neurons exhibited strong N‐terminal copeptin immunoreactivity in all three species, a great majority (~90%) of parvocellular neurons that expressed NPII was devoid of copeptin immunoreactivity in the mouse, and in approximately half (~53%) of them in the rat, whereas in monkey hypothalamus, virtually all NPII‐immunoreactive parvocellular neurons contained strong copeptin immunoreactivity. Immunoelectron microscopy in the mouse clearly showed copeptin‐immunoreactivity co‐localized with NPII‐immunoreactivity in neurosecretory vesicles in the internal layer of the ME and posterior pituitary, but not in the external layer of the ME. Intracerebroventricular administration of a prohormone convertase inhibitor, hexa‐d‐arginine amide resulted in a marked reduction of copeptin‐immunoreactivity in the NPII‐immunoreactive magnocellular PVN neurons in the mouse, suggesting that low protease activity and incomplete processing of pro‐AVP could explain the disproportionally low levels of N‐terminal copeptin expression in rodent AVP (NPII)‐expressing parvocellular neurons. Physiologic and phylogenetic aspects of copeptin expression among neuroendocrine neurons require further exploration.
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Keywords | copeptin
hypothalamo‐pituitary–adrenal system
immunohistochemistry
paraventricular nucleus of the hypothalamus
processing
vasopressin
RRID: AB_2722604
RRID: AB_2061966
RRID: AB_2314234
RRID: AB_10013361
RRID: AB_2313960
RRID: AB_2722605
RRID: AB_90782
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Published Date | 2020-09-06
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Publication Title |
Journal of Comparative Neurology
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Volume | volume529
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Issue | issue7
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Publisher | Wiley
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Start Page | 1372
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End Page | 1390
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ISSN | 0021-9967
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NCID | AA00695917
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2020 The Authors.
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File Version | publisher
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PubMed ID | |
DOI | |
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Related Url | isVersionOf https://doi.org/10.1002/cne.25026
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License | https://creativecommons.org/licenses/by/4.0/
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Funder Name |
Japan Agency for Medical Research and Development
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助成番号 | 961149
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