ID | 60133 |
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Author |
Makanae, Aki
Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Tajika, Yuki
Gunma University, Department of Anatomy, Graduate School of Medicine
Nishimura, Koki
Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Saito, Nanami
Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Tanaka, Jun-Ichi
Okayama University Research Core for Interdisciplinary Sciences (RCIS)
Satoh, Akira
Okayama University Research Core for Interdisciplinary Sciences (RCIS)
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Abstract | The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nerve-dependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration. The presence of nerves is an important factor in successful organ regeneration in amphibians. The Mexican salamander, Ambystoma mexicanum, is able to regenerate limbs, tail, and gills when nerves are present. However, the nervedependency of tooth regeneration has not been evaluated. Here, we reevaluated tooth regeneration processes in axolotls using a three-dimensional reconstitution method called CoMBI and found that tooth regeneration is nerve-dependent although the dentary bone is independent of nerve presence. The induction and invagination of the dental lamina were delayed by denervation. Exogenous Fgf2, Fgf8, and Bmp7 expression could induce tooth placodes even in the denervated mandible. Our results suggest that the role of nerves is conserved and that Fgf+Bmp signals play key roles in axolotl organ-level regeneration.
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Keywords | Cell proliferation
Differentiation
Morphogenesis
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Published Date | 2020-06-09
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Publication Title |
Scientific Reports
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Volume | volume10
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Issue | issue1
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Publisher | Nature Research
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Start Page | 9323
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ISSN | 2045-2322
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © The Author(s) 2020
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File Version | publisher
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DOI | |
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Related Url | isVersionOf https://doi.org/10.1038/s41598-020-66142-2
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License | http://creativecommons.org/licenses/by/4.0/
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