ID | 31723 |
JaLCDOI | |
FullText URL | |
Author |
Araki, Shinako
Miyagi, Yasunari
Kawanishi, Kunihiro
Yamamoto, Junko
Yoshinouchi, Mitsuo
Kudo, Takafumi
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Abstract | The in vitro radiosensitizing effects of docetaxel have been reported, but the DNA damage caused by the irradiation after docetaxel exposure has not been investigated. In this study, the authors attempted to evaluate the radiosensitizing effects in terms of cell survival and DNA single-strand breaks in a human ovarian adenocarcinoma cell line (known as line BG-1) and a human cervical squamous cell carcinoma cell line (known as line SiHa). The cell lines were exposed to various concentrations of docetaxel (from 2.27 x 10(-3) to 2.27 microg/ml) to investigate the cytocidal effects by colony-formation assay. DNA single-strand breaks after exposure to 2.27 microg/ml of docetaxel for 30 min or 100 min were measured by the alkaline-elution assay. The remarkable cytotoxicity of docetaxel followed by irradiation was observed when concentrations were greater than 2.27 x 10(-2) microg/ml in both cell lines. The combination of docetaxel and irradiation appears to be supraadditive. The DNA single-strand breaks induced by the irradiation were enhanced in both cell lines (BG-1; P < 0.01, SiHa; P < 0.05). The synergistic cytocidal effect cannot be explained quantitatively only by the single-strand breaks. |
Keywords | docetaxel
DNA single-strand break
radiosensitizer
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 2002-02
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Volume | volume56
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Issue | issue1
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Publisher | Okayama University Medical School
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Start Page | 13
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End Page | 18
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |