ID | 65938 |
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Kato, Soma
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kawata, Kazumi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Nishida, Takashi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Mizukawa, Tomomi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takigawa, Masaharu
Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
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Iida, Seiji
Department of Oral Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Kubota, Satoshi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Abstract | Cellular communication network factor 2 (CCN2) molecules promote endochondral ossification and articular cartilage regeneration, and circular RNAs (circRNAs), which arise from various genes and regulate gene expression by adsorbing miRNAs, are known to be synthesized from CCN2 in human vascular endothelial cells and other types of cells. However, in chondrocytes, not only the function but also the presence of CCN2-derived circRNA remains completely unknown. In the present study, we investigated the expression and function of CCN2-derived circRNAs in chondrocytes. Amplicons smaller than those from known CCN2-derived circRNAs were observed using RT-PCR analysis that could specifically amplify CCN2-derived circRNAs in human chondrocytic HCS-2/8 cells. The nucleotide sequences of the PCR products indicated novel circRNAs in the HCS-2/8 cells that were different from known CCN2-derived circRNAs. Moreover, the expression of several Ccn2-derived circRNAs in murine chondroblastic ATDC5 cells was confirmed and observed to change alongside chondrocytic differentiation. Next, one of these circRNAs was knocked down in HCS-2/8 cells to investigate the function of the human CCN2-derived circRNA. As a result, CCN2-derived circRNA knockdown significantly reduced the expression of aggrecan mRNA and proteoglycan synthesis. Our data suggest that CCN2-derived circRNAs are expressed in chondrocytes and play a role in chondrogenic differentiation.
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Keywords | Chondrocyte
CCN2
Circular RNA
ACAN
Chondrocytic differentiation
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Note | The version of record of this article, first published in Journal of Cell Communication and Signaling, is available online at Publisher’s website: http://dx.doi.org/10.1007/s12079-023-00782-7
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Published Date | 2023-09-11
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Publication Title |
Journal of Cell Communication and Signaling
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Volume | volume17
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Issue | issue4
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Publisher | Springer Science and Business Media LLC
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Start Page | 1501
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End Page | 1515
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ISSN | 1873-9601
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © The Author(s) 2023
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File Version | publisher
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DOI | |
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Related Url | isVersionOf https://doi.org/10.1007/s12079-023-00782-7
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License | http://creativecommons.org/licenses/by/4.0/
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Citation | Kato, S., Kawata, K., Nishida, T. et al. Expression and function of CCN2-derived circRNAs in chondrocytes. J. Cell Commun. Signal. 17, 1501–1515 (2023). https://doi.org/10.1007/s12079-023-00782-7
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | JP19K10109
JP19H03817
JP20K09889
JP20K20611
JP21H03105
JP23K17439
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