| ID | 56096 |
| FullText URL | |
| Author |
Uemura, Ryota
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Ogura, Mikako
Faculty of Agriculture , Okayama University
Matsumaru, Chihiro
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Akiyama, Tsuyoshi
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Maeda, Megumi
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Kaken ID
researchmap
Kimura, Yoshinobu
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Kaken ID
publons
researchmap
|
| Abstract | Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials
|
| Keywords | Acidic PNGase
FNG: free N-glycan
Fuc: L-fucose
Gal: D-galactose
GlcNAc: N-acetyl-D-glucosamine
HPLC: high-performance liquid chromatography
Man: D-mannose
NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc
NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc
NeuNAc: N-acetylneuraminic acid
PA-: pyridylamino
PNGase-A: aPNGase from almond seed
PNGase-Le: aPNGase from tomato (Solanum lycopersium L.)
PNGase: peptide:N-glycanase
PTC: plant complex type
RCA120: Ricinus communis agglutinin (120 kDa)
RP-HPLC: reversed-phase HPLC
SF-HPLC: size-fractionation HPLC
Xyl: D-xylose
affinity chromatography
enzyme assay
free N-glycans
transgenic plant
|
| Published Date | 2018-04
|
| Publication Title |
Bioscience, Biotechnology, and Biochemistry
|
| Volume | volume82
|
| Issue | issue7
|
| Publisher | Taylor & Francis
|
| Start Page | 1172
|
| End Page | 1175
|
| ISSN | 09168451
|
| NCID | AA10824164
|
| Content Type |
Journal Article
|
| language |
English
|
| OAI-PMH Set |
岡山大学
|
| File Version | author
|
| PubMed ID | |
| DOI | |
| Web of Science KeyUT | |
| Related Url | isVersionOf https://doi.org/10.1080/09168451.2018.1459464
|
| Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
|
| 助成番号 | 15K07841
15KK0282
17K0819709
|
